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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/60939
Title: | WRKY transcription factor in sugarcane defence against Colletotrichum falcatum |
Other Titles: | Not Available |
Authors: | Nandakumar M., R. Viswanathan, P. Malathi and A. Ramesh Sundar |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Sugarcane Breeding Institute |
Published/ Complete Date: | 2014-06-25 |
Project Code: | Not Available |
Keywords: | sugarcane, red rot, Colletotrichum falcatum, WRKY TF’s, ScWRKY, FastPCR |
Publisher: | Society for Sugarcane Research and Development, Coimbatore, India |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | In sugarcane, red rot is a major disease caused by Colletotrichum falcatum Went which infects cane stalks. The infected stalks become unfit for milling and quality of juice is affected due to inversion of glucose and fructose. The function of WRKY TF’s in defense is proven as several EST-contigs were identified. Also a ScWRKY gene was identified for its active role in defence response against Sporisorium scitamineum, salicylic acid, drought and salinity. Among five different transcription factors studied in sugarcane during C. falcatum interaction, WRKY showed differential expression pattern in resistant and susceptible varieties.In continuation of the previous studies, we have taken up detailed studies to identify active involvement of WRKY TF’s during sugarcane and C. falcatum interaction. A total of 36 ScWRKY sequences were retrieved and primers were designed using FastPCR tool (Ver. 3.6.62). Expression analyses were performed with the sugarcane cultivars Co 93009(R) and CoC 671(S) which were inoculated with C. falcatum pathotype Cf671 conidial suspension (1x106/mL-1) and the samples were collected at 0, 6, 12, 24, 36, 48 and 72h later. RNA was isolated from the samples and expression studies were taken up by RT-PCR. Among the 36 ScWRKY primers, 16, 18, 19, 26, 31 and 35 primers showed expression and they were further used to study the spatial and temporal expression pattern during C. falcatum interaction with sugarcane. WRKY 16, 18 and19 showed higher expression in susceptible cultivar at 48h where as it was expressed low in other intervals. However the resistant cultivar showed early and late induction. WRKY 26 expressed in early stages in both susceptible and resistant samples. Also WRKY 31 and 35 showed high expression in the susceptible cultivar compared to the resistant cultivar. Further studies are being continued to identify specific expression of resistance associated transcription factor in WRKY family and their relation to expression of genes involved in defence related signal transduction pathway |
Description: | Not Available |
ISBN: | Not Available |
Type(s) of content: | Proceedings |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Not Available |
Volume No.: | Not Available |
Page Number: | 237-239 |
Name of the Division/Regional Station: | Division of crop protection |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/60939 |
Appears in Collections: | CS-SBI-Publication |
Files in This Item:
File | Description | Size | Format | |
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24. Nanda 2014.pdf | 983.92 kB | Adobe PDF | View/Open |
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