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  2. Fisheries A6
  3. ICAR-Central Marine Fisheries Research Institute I7
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Please use this identifier to cite or link to this item: http://krishi.icar.gov.in/jspui/handle/123456789/63118
Title: Differential responses of sexual and asexual Artemia to genotoxicity by a reference mutagen:Is the comet assay a reliable predictor of population level responses?
Other Titles: Not Available
Authors: Sukumaran,Sandhya
Grant,Alastair
ICAR Data Use Licennce: Not Available
Author's Affiliated institute: Not Available
Published/ Complete Date: 2013
Project Code: Not Available
Keywords: Artemia franciscana
Artemia parthenogenetica
Ethyl methanesulfonate
Genotoxicity
Comet assay
Fitness effects
Citation: Not Available
Series/Report no.: Not Available
Abstract/Description: The impactofchronicgenotoxicitytonaturalpopulationsisalwaysquestionedduetotheir reproductivesurplus.WeusedacometassaytoquantifyprimaryDNAdamageafterexposuretoa referencemutagenethylmethanesulfonateintwospeciesofcrustaceanwithdifferentreproductive strategies(sexual Artemia franciscana and asexual Artemia parthenogenetica). Wethenassessedwhether this predictedindividualperformanceandpopulationgrowthrateoverthreegenerations. Artemia were exposedtodifferentchronicconcentrations(0.78mM,1.01mM,1.24mMand1.48mM)ofethyl methanesulfonatefrominstar1onwardsfor3h,24h,7days,14daysand21daysandpercentagetail DNA valueswereusedforcomparisonsbetweenspecies.ThepercentagetailDNAvaluesshowed consistentlyelevatedvaluesupto7daysandshowedareductionfrom14daysonwardsin A. franciscana. Whilstin A. parthenogenetica such areductionwasevidenton21daysassessment.The values ofpercentagetailDNAafter21dayswerecomparedwithpopulationlevelfitnessparameters, growth,survival,fecundityandpopulationgrowthratetoknowwhetherprimaryDNAdamageas measuredbycometassayisareliablebiomarker.SubstantialincreaseintailDNAvalueswasassociated with substantialreductionsinallthefitnessparametersintheparentalgenerationof A. franciscana and parental,F1andF2generationsof A. parthenogenetica. Socometresultsweremorepredictiveinasexual speciesovergenerations.Theseresultspointedtotheimportanceofpredictingbiomarkerresponses from multigenerationalconsequencesconsideringlifehistorytraitsandreproductivestrategiesin ecologicalriskassessments.
Description: Not Available
ISSN: Not Available
Type(s) of content: Article
Sponsors: Not Available
Language: English
Name of Journal: Ecotoxicology and EnvironmentalSafety
Volume No.: 91
Page Number: 110-116
Name of the Division/Regional Station: Not Available
Source, DOI or any other URL: http://eprints.cmfri.org.in/9638/1/ecotoxicology_and_environmental_safety_paper.pdf
URI: http://krishi.icar.gov.in/jspui/handle/123456789/63118
Appears in Collections:FS-CMFRI-Publication

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