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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/64689
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Pandey, S. Gulati, S., Goyal, E., Singh, S., Kumar, K., Nain, L. and Saxena, A.K | en_US |
dc.date.accessioned | 2021-09-25T16:06:07Z | - |
dc.date.available | 2021-09-25T16:06:07Z | - |
dc.date.issued | 2016-01-01 | - |
dc.identifier.citation | Not Available | en_US |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/64689 | - |
dc.description | Not Available | en_US |
dc.description.abstract | A metagenomic library consisting of 7500 clones was constructed from the soil continuously irrigated with effluents of paper and pulp mill. A cellulase positive clone H-10 was identified by function based screening. A β-1, 4- endoglucanase gene with an open reading frame of 1499 bp was identified from this positive clone. The deduced amino acid sequence analysis revealed that amplified β-1, 4-endoglucanase belongs to GH5 and shares 98% similarity with Bacillus subtilis (EF070194), 95% with Bacillus subtilis IARI-SP-1(KF240847) 96% with B. subtilis (AY183475) and 95% with uncultured bacterium clone (JX567739). Clone H-10 indicated higher CMCase activity of 1 IU/ml on the substrate CMC as compared to rice straw (0.5 IU/ml). The characterization of enzymes revealed its pH optima between 8.5–9 and retained 80% of activity at pH 11. The thermal profile of the enzyme indicated broad range of thermotolerance. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Not Available | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Metagenomic libraryCellulaseFunction based screeningβ-1, 4-endoglucanasePaper and pulp mill | en_US |
dc.title | Construction and screening of metagenomic library derived from soil for β-1, 4-endoglucanase gene | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Biocatalysis and Agricultural Biotechnology | en_US |
dc.publication.volumeno | 5 | en_US |
dc.publication.pagenumber | 186-192 | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | Not Available | en_US |
dc.publication.authorAffiliation | ICAR-NATIONAL BUREAU OF AGRICULTURALLY IMPORTANT MICROORGANISMS KUSHMAUR MAU NATH BHANJAN 275103 | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
Appears in Collections: | CS-NBAIMO-Publication |
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