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http://krishi.icar.gov.in/jspui/handle/123456789/64767| Title: | Cryoinjury in relation to cholesterol content of bull spermatozoa |
| Other Titles: | Not Available |
| Authors: | Srivastava N, Srivastava S K Ghosh S K, Kumar A Jerome A, MAHAK SINGH Kumar A |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Research Complex for NEH Region ICAR::Indian Veterinary Research Institute |
| Published/ Complete Date: | 2015-01-01 |
| Project Code: | Not Available |
| Keywords: | Cholesterol, Cryocapacitation Cryoinjury Pre-freeze Spermatozoa |
| Publisher: | Not Available |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Though semen cryopreservation has many advantages, it decreases tolerance of spermatozoa to stress of temperature variations, thus disposing spermatozoa to increased cryoinjury. Predicting cryoinjury to spermatozoa is difficult with available laboratory protocols. The present study was designed to evaluate the cryoinjury prediction of sperm in relation to its cholesterol content. The cholesterol content at fresh, pre-freeze and post-thaw stage was 21.95±0.97, 9.58±0.45 and 7.44±0.44 ìg/100 million spermatozoa. Semen quality parameters (SQP) at pre-freeze and post-thaw stages were individual motility (71.58±1.29 vs. 47.83±1.16), acrosome integrity (71.00±1.48 vs. 61.58±1.70), Chlortetracycline (CTC) pattern (F: 29.67±1.14 vs. 15.83±1.02; B: 48.83±2.06 vs. 53.58±1.27; AR: 21.50±1.84 vs. 30.58±1.85). Study indicated that cholesterol content of fresh spermatozoa could be used to predict cholesterol content of spermatozoa at pre-freeze and post-thaw stages with medium to high level of accuracy. This study revealed strong evidence that cholesterol content of pre-freeze bull spermatozoa could be a good predictor of level of cryoinjury following preservation at ultra low temperature. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Indian Journal of Animal Research |
| Journal Type: | Peer Reviewed |
| NAAS Rating: | 6.4 |
| Volume No.: | 49 |
| Page Number: | 308-312 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/64767 |
| Appears in Collections: | NRM-RCNR-Publication |
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