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http://krishi.icar.gov.in/jspui/handle/123456789/6684
Title: | Culture, characterization and differentiation of cells from buffalo (Bubalus bubalis) amnion |
Other Titles: | Not Available |
Authors: | A. Mann, R. P. Yadav, J. Singh, D. Kumar, B. Singh and P. S. Yadav |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR-Central Soil Salinity Research Institute, Karnal |
Published/ Complete Date: | 2013-07-01 |
Project Code: | Not Available |
Keywords: | Amnion cells, Buffalo, Differentiation, Pluripotency, Stem cells |
Publisher: | Springer |
Citation: | Mann A, R. P. Yadav, J. Singh, D. Kumar, B. Singh and P. S. Yadav. (2013) Culture, characterization and differentiation of cells from buffalo (Bubalus bubalis) amnion. Cytotechnology. 65(1):23-30 |
Series/Report no.: | Not Available; |
Abstract/Description: | Stem cells present an important tool in livestock assisted reproduction and veterinary therapeutic field such as tissue engineering. We report for the first time isolation of pluripotent stem cell-like cells expressing pluripotency markers (alkaline phospahatase, OCT-4, NANOG and SOX-2) from the amnion of water buffalo (Bubalus bubalis). The cells showed no apparent abnormalities in their chromosomal profiles before and after cryopreservation. The cytochemical staining revealed that pluripotent cells were capable of undergoing directed differentiation in vitro into osteocytes. It could be inferred that amnionderived pluripotent stem cell-like cells can be isolated, cultured for many passages and differentiated into mesodermlineage, and may be an alternative source to mesenchymal stem cells. These cells can have applications in assisted reproduction, developmental biological and regenerative medicine |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Cytotechnology |
NAAS Rating: | 7.78 |
Volume No.: | 65 |
Page Number: | 23–30 |
Name of the Division/Regional Station: | Crop Improvement |
Source, DOI or any other URL: | DOI 10.1007/s10616-012-9464-z |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/6684 |
Appears in Collections: | NRM-CSSRI-Publication |
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