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  2. Fisheries A6
  3. ICAR-Central Institute of Fisheries Education I4
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Please use this identifier to cite or link to this item: http://krishi.icar.gov.in/jspui/handle/123456789/67129
Title: Bicistronic DNA vaccine macromolecule complexed with poly lactic-co-glycolic acid-chitosan nanoparticles enhanced the mucosal immunity of Labeo rohita against Edwardsiella tarda infection.
Authors: Tasok Leya
Irshad Ahmad
Rupam Sharma
Gayatri Tripathi
K. Pani Prasad
K.V.Rajendran
Megha kadam Bedikar
Published/ Complete Date: 2020-4-11
Keywords: Mucosal immunity,Bicistronic DNA vaccine,Poly lactic-co-glycolic acid-chitosan nanoparticles,Labeo rohita,Edwardsiella tarda
Publisher: Not Available
Citation: Leya, T., Ahmad, I., Sharma, R., Tripathi, G., Kurcheti, P.P., Rajendran, K.V., Bedekar, M.K., 2020. Bicistronic DNA vaccine macromolecule complexed with poly lactic-co-glycolic acid-chitosan nanoparticles enhanced the mucosal immunity of Labeo rohita against Edwardsiella tarda infection. Internatio
Abstract/Description: DNA vaccine is an important tool to elicit both humoral and cellular immunity. Present study investigates mucosal immune response of Labeo rohita (15 ? 04 g) to plasmid DNA (pDNA) vaccine macromolecule complexed with nanoparticles (NPs). Poly lactic-co-glycolic acid (PLGA), Chitosan (Chit) and PLGA-Chit-NPs were synthesized by double emulsion solvent evaporation method. Synthesized NPs were complexed with pDNA (pGPD + IFN) vaccine construct. Size and zeta potential of PLGA-NPs, Chit-NPs and PLGA-Chit-NPs-pDNA complex were recorded to be 120 nm and +0.5 mV, 117 nm and +32 mV, 189 nm and +11 mV, respectively. Immunization by immersion was carried out in two groups receiving PLGA-Chit-NPs-pDNA (T1) and PLGA-NPs-pDNA (T2) respectively. After immersion, samples were collected on 0, 2, 4, 7, 15 and 30 days from mucosa-associated lymphoid tissues (MALT) for mRNA expression studies of IgM, IgD and IgZ using qRT-PCR. Significant up-regulation of the mRNA expression of IgM, IgD, and IgT were observed in MALT in immunized fish compared to control. After 30 days post-immunization fish were infected with a virulent strain of Edwardsiella tarda. The highest relative percentage survival was observed in T1 (64.7%) compared to T2. The study showed better efficiency of pDNA-PLGA-Chit-NPs compared to pDNA-PLGA-NPs for inducing adaptive mucosal immunity in fish.
Description: Not Available
ISBN: Not Available
ISSN: 0141-8130
Type(s) of content: Research Paper
Language: English
Name of Journal: International Journal of Biological Macromolecules.
URI: http://krishi.icar.gov.in/jspui/handle/123456789/67129
Appears in Collections:FS-CIFE-Publication

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