KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/67878
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Viswanathan, R., A. Ramesh Sundar, P. Malathi, V. Ganesh Kumar, P.R. Rahul and P.T. Pratima | en_US |
dc.date.accessioned | 2021-11-27T08:50:22Z | - |
dc.date.available | 2021-11-27T08:50:22Z | - |
dc.date.issued | 2010-12-27 | - |
dc.identifier.citation | Not Available | en_US |
dc.identifier.isbn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/67878 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Red rot of sugarcane caused by Colletotrichum falcatum Went is one of the devastating diseases of sugarcane causing significant loss to sugarcane production in the country. Complex polyploidy and lack of information on inheritance to red rot in sugarcane make breeding for red rot resistance more difficult. Hence researchers studied the mechanism of red rot resistance in sugarcane in detail. Initial studies based on biochemical tools identified oxidative enzymes and red rot pigments in disease resistance. Further studies revealed the role of pathogenesis-related (PR) proteins and 3-deoxyanthocyanidin phytoalexins especially luteolinidin and apigeninidin in red rot resistance. Recent studies using semiquantitative RT-PCR after pathogen inoculation from sugarcane cultivars varying in red rot resistance, revealed differential accumulation of transcripts of the flavanoid biosynthetic pathway like coumarate-4-hydroxylase, chalcone synthase, chalcone reductase, flavanoid 3`-5` hydroxylase and flavanoid glycosyl transferase and this transcript analysis, further confirmed the role of sugarcane phytoalexins in red rot resistance. Similarly the role of PR- proteins like chitinase and -1,3-glucanase was established at the transcript level. Detailed molecular studies using differential display (DD)-RT-PCR identified expression of more number of differentially expressed transcripts during the host-pathogen interaction. Full length sequences of many potential transcripts were identified and are being characterized. Also to identify specific proteins involved in host resistance, proteomic approach has been attempted by optimizing sample preparation from stalk tissues, 2-D electrophoresis (2-DE), downstream processing of identified spots and bioinformatics. Several resistance associated proteins spots were identified and they are being analysed critically | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Society for Promotion of Horticulture, IIHR. | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | sugarcane, Colletotrichum falcatum, resistance, 3-deoxyanthocyanidin phytoalexins , semiquantitative RT-PCR, 2-D electrophoresis | en_US |
dc.title | New insights into molecular basis of red rot resistance in sugarcane | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Proceedings | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Not Available | en_US |
dc.publication.volumeno | Not Available | en_US |
dc.publication.pagenumber | 123 | en_US |
dc.publication.divisionUnit | Division of crop protection | en_US |
dc.publication.sourceUrl | Not Available | en_US |
dc.publication.authorAffiliation | ICAR::Sugarcane Breeding Institute | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
Appears in Collections: | CS-SBI-Publication |
Files in This Item:
File | Description | Size | Format | |
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IIHR 2010.pdf | 1.2 MB | Adobe PDF | View/Open |
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