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http://krishi.icar.gov.in/jspui/handle/123456789/67955
Title: | First report of maize yellow mosaic virus (MaYMV) infecting sugarcane and maize in India and its molecular characterization |
Other Titles: | Not Available |
Authors: | Viswanathan R., K. Nithya, J. VishnuVardhan, S. Balasaravanan, D.Vishalakshi1 and K. Kaverinathan |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Sugarcane Breeding Institute |
Published/ Complete Date: | 2021-06-19 |
Project Code: | Not Available |
Keywords: | Maize yellow mosaic virus (MaYMV) , RT-PCR assays , MaYMV specific primers |
Publisher: | Not Available |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Based on the recent reports of widespread occurrence of maize yellow mosaic virus (MaYMV) in many of the agriculturally important Poaceae crops in different countries, we suspected the possible occurrence of the virus in India. During 2020, we analyzed 10 maize samples showing mosaic like symptoms grown nearby sugarcane fields in Coimbatore and Namakkal districts of Tamil Nadu and 106 sugarcane samples from different varieties showing mosaic and yellow leaf symptoms for MaMYV infection. RT-PCR assay revealed that all the maize leaf samples and 14.15% (15/106) of the sugarcane leaf samples were found to be infected with MaYMV which was further confirmed by direct sequencing of the amplified products by Sanger method at both the ends. The pairwise multiple sequence alignment of all the 16 consensus sequences of this study from sugarcane and maize has clearly revealed the highest nucleotide similarities with 97.4 % to 100% among themselves as well as to other MaYMV sequences of maize from Africa (Tanzania, South Africa, Ethiopia, Kenya, South Sudan and Burkino Faso) and China and all the Saccharum isolates from China. The MaYMV isolate from Saccharum hybrid CP 81-1384 had shown the highest similarity of 100% with MaYMV of both the crops irrespective of the geographic regions whereas, Saccharum hybrid LG 08478 isolate had shown 96.5% to 97.8% identity only with other MaYMV isolates. In phylogenetic analysis, all the 45 isolates sequences (15 from sugarcane varieties and one from maize in this study and the remaining 29 retrieved from genbank source representatively taken from the reported countries) had clustered together in a single clade. However, host crop based sub clades were observed from different countries as most of the Zea mays MaYMV isolates sub clustered together irrespective of countries/ geographical regions and most of the MaYMV from sugarcane from this study as well as from China sub clustered separately. Our research findings clearly showed that presence of MaYMV in India in maize and sugarcane for the first time. Also, mixed infection of MaYMV with other sugarcane viruses predominantly infecting the crop was also recorded. Since mixed infection of different viruses in sugarcane such as SCMV, SCSMV and ScYLV are a common phenomenon and both ScYLV and MaYMV belonged to the genus Polerovirus, its mixed infection status and the kind of synergistic effect in symptom expression is to be studied further at large scale in future |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Proceedings |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Not Available |
Volume No.: | Not Available |
Page Number: | 578-581 |
Name of the Division/Regional Station: | Division of crop protection |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/67955 |
Appears in Collections: | CS-SBI-Publication |
Files in This Item:
File | Description | Size | Format | |
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20. MaYMV CaneCon2021.pdf | 183.6 kB | Adobe PDF | View/Open |
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