Skip navigation
DSpace logo
  • Home
  • Browse
    • SMD
      & Institutes
    • Browse Items by:
    • Published/ Complete Date
    • Author/ PI/CoPI
    • Title
    • Keyword (Publication)
  • Sign on to:
    • My KRISHI
    • Receive email
      updates
    • Edit Profile
ICAR logo

KRISHI

ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)


  1. KRISHI Publication and Data Inventory Repository
  2. Crop Science A5
  3. ICAR-Sugarcane Breeding Institute H9
  4. CS-SBI-Publication
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item: http://krishi.icar.gov.in/jspui/handle/123456789/68214
Title: Biochemical markers of pollen stigma interaction in sugarcane (Saccharum spp)
Other Titles: Not Available
Authors: S.Alarmelu and R.M.Shanthi
ICAR Data Use Licennce: http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf
Author's Affiliated institute: ICAR::Sugarcane Breeding Institute
Published/ Complete Date: 2013-06-28
Project Code: Not Available
Keywords: Self incomptability, Sugarcane, Pollen study
Publisher: Society for Applied Biotechnology ( India )
Citation: Alarmelu S and R.M.Shanthi ., 2013
Series/Report no.: Not Available;
Abstract/Description: Self incompatibility occurs in greater frequency among the members of gramineae and controlled by two loci system. Sugarcane as any other polyploid crop with high level of heterozygosity show gametophytic incompatibility which is of special interest to breeders since it encourages outcrossing, prohibits selfing and contributes to propagation of species. Enzymes detected in sugarcane pollen have been found to play a crucial role in the control of incompatibility and among them esterase isozyme has been shown to be of both sporophytic and gametophytic origin and provide a source of markers for studying development of pollen pistil interaction The present study reports a biochemical approach to identify isozyme markers that control incompatibility reaction operating in sugarcane. Two isoenzyme systems, namely, esterase and glutamate dehydrogenase were studied to assess the variation in the protein content and their expression in the pistils, pollen and stigma of compatible and incompatible sugarcane clones that are used as parents in breeding programmes. Esterase and glutamate dehydrogenase isozyme patterns were studied in the anthers of six pollen parents and stigmas of two pistil parents. In general, the anther esterase pattern of Co 86011, Co 62198, Co 91004, Co 99002 and Co 1148 on the stigmas of Co 88028 indicated qualitative differences among them. Co 86011 had one intense band which was not present in other pollen parents might be the cause for the compatible reaction in crosses effected using Co 86011 as pollen parent. Esterase activity was found to be high in Co 86011 pollen on Co 88028 stigma in comparison with Co 88025 stigma and pollen of Co 62198, Co 91004 and Co 99002. Three fast migrating GDH bands were detected in the anther and stigma extracts of the parents studied. The isozyme pattern of GDH indicated that GDH 2 was common for pollen and pistil parents, GDH 1 found only in self incompatible parents viz., Co 62198, Co 91004 and Co 99002 and GDH 3 only in the compatible parent Co 86011. The presence of esterases and its activity suggest that pollen esterases complexes with stigma enables pollen tube entry into the stigma and similarly the high esterase activity in the mature stigma of compatible parents suggests a possible role for these enzymes in compatible and GDH for incompatible pollinations.
Description: Not Available
Type(s) of content: Article
Sponsors: Not Available
Language: English
Name of Journal: First International and Third National Conference on Biotechnology, Bioinformatics and Bioengineering
Journal Type: Conference
Volume No.: Not Available
Page Number: 25
Name of the Division/Regional Station: Coimbatore
Source, DOI or any other URL: Society for Applied Biotechnology (India)
URI: http://krishi.icar.gov.in/jspui/handle/123456789/68214
Appears in Collections:CS-SBI-Publication

Files in This Item:
File Description SizeFormat 
Tirupathi Conference.pdf7.51 MBAdobe PDFView/Open
Show full item record


Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.

  File Downloads  

Feb 2023: 27481 Jan 2023: 163488 Dec 2022: 133147 Nov 2022: 119666 Oct 2022: 99600 Sep 2022: 107963

Total Download
3680915

(Also includes document to fetched through computer programme by other sites)
( From May 2017 )

ICAR Data Use Licence
Disclaimer
©  2016 All Rights Reserved  • 
Indian Council of Agricultural Research
Krishi Bhavan, Dr. Rajendra Prasad Road, New Delhi-110 001. INDIA

INDEXED BY

KRISHI: Inter Portal Harvester

DOAR
Theme by Logo CINECA Reports

DSpace Software Copyright © 2002-2013  Duraspace - Feedback