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http://krishi.icar.gov.in/jspui/handle/123456789/68541| Title: | Analytical validation of a modified turbidimetric assay to screen sulphur oxidizing bacteria |
| Other Titles: | Not Available |
| Authors: | Amala P.V Sumithra T.G K.J. Reshma F. Anju Shinoj Subramannian P. Vijayagopal |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Central Marine Fisheries Research Institute |
| Published/ Complete Date: | 2020-07-07 |
| Project Code: | Not Available |
| Keywords: | SOB; Thiosulphate; Sulphate concentration; Calibration curve; Spectrometry; Microtitre plate |
| Publisher: | Elsevier |
| Citation: | P.V. Amala, T.G. Sumithra, K.J. Reshma, F. Anju, Shinoj Subramannian, P. Vijayagopal, Analytical validation of a modified turbidimetric assay to screen sulphur oxidizing bacteria, Journal of Microbiological Methods, Volume 176, 2020, 105998, ISSN 0167-7012, |
| Abstract/Description: | Abstract: Conventional turbidimetric assay for sulphate determination was modified to 100 times lesser reaction volume on a convenient format using microtitre plate based platform, targeting routine microbiological applications to screen sulphur oxidizing bacteria (SOB) cultures. The modified assay was linear up to 1500 mg/L of sulphate concentration, which is about 37.5 times more than that of conventional assay. Upon regression analysis, linear equation y = 1.243× + 0.011 was obtained having R2 value of 0.998. The modified assay was fully validated in terms of precision, limit of detection (LOD), limit of quantification (LOQ), sensitivity, selectivity and robustness to assure the reliability during final applications. LOD and LOQ were found as 7.4 mg/L and 24.8 mg/L of sulphate concentration respectively. Further, accuracy of the assay over routine SOB screening media components was tested, and proved as reliable and suitable for the intended application. |
| Description: | Not Available |
| Gov't Doc #: | https://doi.org/10.1016/j.mimet.2020.105998 |
| ISSN: | 0167-7012 |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Journal of Microbiological Methods |
| Journal Type: | online |
| NAAS Rating: | na |
| Impact Factor: | na |
| Volume No.: | 176 |
| Page Number: | Not Available |
| Name of the Division/Regional Station: | ICAR-Krishi Vigyan Kendra Ernakulam, Narakkal PO |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/68541 |
| Appears in Collections: | AExt-ATARI Z11-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Final article after proof SOB.pdf | 552.1 kB | Adobe PDF | View/Open |
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