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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/70236
Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Sowmya Poosapati | en_US |
dc.contributor.author | Prasad Durga Ravulapalli | en_US |
dc.contributor.author | Dinesh Kumar Viswanathaswamy | en_US |
dc.contributor.author | Monica Kannan | en_US |
dc.date.accessioned | 2022-03-15T09:02:08Z | - |
dc.date.available | 2022-03-15T09:02:08Z | - |
dc.date.issued | 2021-11-24 | - |
dc.identifier.citation | Poosapati, S.; Ravulapalli, P.D.; Viswanathaswamy, D.K.; Kannan, M. Proteomics of Two Thermotolerant Isolates of Trichoderma under High-Temperature Stress. J. Fungi 2021, 7, 1002. https:// doi.org/10.3390/jof7121002 | en_US |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/70236 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Several species of the soil borne fungus of the genus Trichoderma are known to be versatile, opportunistic plant symbionts and are the most successful biocontrol agents used in today’s agri culture. To be successful in field conditions, the fungus must endure varying climatic conditions. Studies have indicated that a high atmospheric temperature coupled with low humidity is a major factor in the inconsistent performance of Trichoderma under field conditions. Understanding the molecular modulations associated with Trichoderma that persist and deliver under abiotic stress conditions will aid in exploiting the value of these organisms for such uses. In this study, a com parative proteomic analysis, using two-dimensional gel electrophoresis (2DE) and matrix-assisted laser desorption/time-of-flight (MALDI-TOF-TOF) mass spectrometry, was used to identify proteins associated with thermotolerance in two thermotolerant isolates of Trichoderma: T. longibrachiatum 673, TaDOR673 and T. asperellum 7316, TaDOR7316; with 32 differentially expressed proteins being iden tified. Sequence homology and conserved domains were used to identify these proteins and to assign a probable function to them. The thermotolerant isolate, TaDOR673, seemed to employ the stress signaling MAPK pathways and heat shock response pathways to combat the stress condition, whereas the moderately tolerant isolate, TaDOR7316, seemed to adapt to high-temperature conditions by reducing the accumulation of misfolded proteins through an unfolded protein response pathway and autophagy. In addition, there were unique, as well as common, proteins that were differentially expressed in the two isolates studied. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | MDPI, Basel, Switzerland | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Trichoderma | en_US |
dc.subject | thermotolerance | en_US |
dc.subject | cell wall remodeling | en_US |
dc.subject | MAPK | en_US |
dc.subject | Hsf1 | en_US |
dc.subject | UPR | en_US |
dc.subject | autophagy | en_US |
dc.title | Proteomic analysis reveals different sets of proteins expressed during high temperature stress in two thermotolerant isolates of Trichoderma. | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | NAIP C 2082 | en_US |
dc.publication.journalname | Journal of Fungi | en_US |
dc.publication.volumeno | 7 | en_US |
dc.publication.pagenumber | 2-20 | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | https://doi.org/10.3390/jof7121002 | en_US |
dc.publication.authorAffiliation | ICAR::Indian Institute of Oilseeds Research | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.naasrating | 11.82 | en_US |
Appears in Collections: | CS-IIOR-Publication |
Files in This Item:
File | Description | Size | Format | |
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JoF-trichoderma proteome.pdf | 3.07 MB | Adobe PDF | View/Open |
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