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http://krishi.icar.gov.in/jspui/handle/123456789/7148
Title: | A rapid method for authentication of Buffalo (Bubalus bubalis) meat by alkaline lysis method of DNA extraction and specie specific polymerase chain reaction. |
Other Titles: | Not Available |
Authors: | Not Available Girish P.S, Hunshi S, Vaithiyanathan S, Rajitha R and Ramakrishna C |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::National Research Centre on Meat, Hyderabad |
Published/ Complete Date: | 2013-12-31 |
Project Code: | Not Available |
Keywords: | rapid authentication Buffalo Bubalus Bubalis meat alkaline lysis DNA extraction specie polymerse chain reaction |
Publisher: | Springer Link |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Buffalo (Bubalus bubalis) meat is a major item of export from India but export of beef i.e. meat from cattle (Bos indicus) is prohibited. Also, adulteration of buffalo meat with that of beef (meat from cattle) is a common fraudulent practice because of prohibition on cow slaughter in most states of India. Food analysts require precise identification techniques to implement such regulations. In the present study, a method of DNA extraction by Alkaline lysis from meat samples and speciation of buffalo meat using species specific Polymerase Chain Reaction (PCR) has been reported. Alkaline lysis technique is a rapid method which involves triturating meat with four volumes of 0.2N NaOH, dilution of resultant liquid extract with eight volumes of 0.2N NaOH, heating the mix 75 °C for 20 min followed by neutralization with eight volumes of 0.04N Tris HCl. Entire procedure of DNA extraction takes less than 30 min and it is economical as it involves less expensive chemicals. Method was successfully applied in animal byproducts also viz., liver, heart and kidney. For authentication of buffalo meat, pair of primers was designed based on mitochondrial D loop gene nucleotide sequence. PCR amplification using the designed primers gave amplicon of size 482 bp in buffalo and no amplification was detected in closely related species viz., cattle, sheep and goat meat samples. Results of the assay were highly repetitive and reliable. An export sample referred by export regulation authorities was also analyzed by using the Alkaline lysis method of DNA extraction and species specific PCR which enabled authentication of meat within 5 h. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Article |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Journal of Food Science and Technology |
NAAS Rating: | 7.95 |
Volume No.: | 50(I) |
Page Number: | 141-146 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | https://doi.org/10.1007/s13197-011-0230-6 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/7148 |
Appears in Collections: | AS-NRCMeat-Publication |
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