KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/71573
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Mohanty NN | en_US |
dc.contributor.author | Hemadri D | en_US |
dc.contributor.author | Munivenkatarayappa A | en_US |
dc.contributor.author | Shetty N | en_US |
dc.contributor.author | Subramanyam V | en_US |
dc.contributor.author | Biswas SK | en_US |
dc.contributor.author | Chanda MM | en_US |
dc.contributor.author | Shivachandra SB | en_US |
dc.date.accessioned | 2022-04-18T09:25:06Z | - |
dc.date.available | 2022-04-18T09:25:06Z | - |
dc.date.issued | 2021-01-04 | - |
dc.identifier.citation | Mohanty NN, Hemadri D, Munivenkatarayappa A, Shetty N, Subramanyam V, Biswas SK, Chanda MM, Shivachandra SB. Development of recombinant NS1-NS3 antigen based indirect ELISA for detection of bluetongue antibodies in sheep. J Immunol Methods. 2021 Mar;490:112959. doi: 10.1016/j.jim.2021.112959. Epub 2021 Jan 4. PMID: 33412173. | en_US |
dc.identifier.issn | 0022-1759 | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/71573 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Bluetongue is an insect borne (Culicoides) viral disease of small ruminants. The virus blankets the globe with a wide serotypic variation, numbered from 1 to 28. In India 21 different serotypes have been reported to be circulating across the various agro-climatic zones of the country. Non-structural proteins (NSPs) of bluetongue virus have always remained ideal target for differentiation of infected from vaccinated animals. The current study is an extrapolation of our previous work where a novel fusion construct comprising of bluetongue viral segment NS1 and NS3 was successfully cloned, expressed, purified with an efficient strategy for its suitable implementation as a diagnostic antigen. In this study, the applicability of the fusion construct has been further evaluated and optimised for field applicability. The fusion construct used in an ELISA platform projected a relative diagnostic sensitivity and specificity of 98.1% and 95.5% respectively against a pre-established test panel. The rNS1-NS3 ELISA showed substantially good agreement with the commercial BTV antibody detection kit. Finally, the study brings together the diagnostic capability of two NSPs, which can be a handy tool for sero-surveillance of bluetongue. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Elsevier Publishing | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Bluetongue | en_US |
dc.subject | Diagnosis | en_US |
dc.subject | Indirect ELISA | en_US |
dc.subject | Non-structural protein | en_US |
dc.subject | Sero-surveillance | en_US |
dc.title | Development of recombinant NS1-NS3 antigen based indirect ELISA for detection of bluetongue antibodies in sheep. | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Journal of Immunological Methods | en_US |
dc.publication.volumeno | 490 | en_US |
dc.publication.pagenumber | 1-6 | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | 10.1016/j.jim.2021.112959 | en_US |
dc.publication.authorAffiliation | National Institute of Veterinary Epidemiology and Disease Informatics | en_US |
dc.publication.authorAffiliation | ICAR-Indian Veterinary Research Institute, Mukteswar-Kumaon, Nainital 243122, Uttarakhand, India. | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.journaltype | Peer reviewer journal | en_US |
dc.publication.naasrating | 7.9 | en_US |
dc.publication.impactfactor | 2.303 | en_US |
Appears in Collections: | AS-NIVEDI-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.