First report on serological evidence of bovine viral diarrhea virus (BVDV) infection in farmed and free ranging mithuns (Bos frontalis).

Abstract

Despite reports of BVDV infection in several domestic and wild ruminants, no information exists for mithun (Bos frontalis) species. Hence, this study was undertaken to determine prevalence of BVDV infection in mithuns, which contribute significantly to local economy in the North Eastern region of India. Blood and serum samples were collected between 2013 and 2016 from mithuns (n = 466) belonging to the states of Nagaland, Mizoram, and Arunachal Pradesh. Serum samples were tested for BVDV antibodies by a commercial ELISA and leukocytes were tested for BVDV by real-time RT-PCR. The overall true seroprevalence rate was 13.1% (95% confidence interval, CI: 6.9-17.8%) with higher prevalence in mithuns reared under semi-intensive system (27.5%) than in free-ranging mithuns (7.6%). Among the three states, seroprevalence (16.2%) was highest in Nagaland, while prevalence rates varied markedly among geographical locations. Age-wise data showed highest seroprevalence rate in >6-year-old animals (20.6%) than 2-6 years old (16.9%), 6 months-2 years old (8.5%), and <6-month-old animals (11.3%). The seroprevalence was higher in males (20.9%) than in females (12.1%). Among the four mithun strains, higher prevalence was evident in Manipur (30.3%) than Arunachal (21.3%), Nagaland (11.7%), and Mizoram strain (10.2%). However, no BVDV genomic RNA could be detected. The results provide first serological evidence of BVDV infection in mithun species and extend the knowledge on BVDV host range. The baseline data will help further investigations on epidemiology of BVD in mithun and its impact on mithun production.