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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/73596
Title: | An expedient ion chromatography based method for high‐ throughput analysis of phytic acid in groundnut kernels |
Other Titles: | Not Available |
Authors: | Aman Verma, Sushmita Singh, Lokesh K. Thawait, Mahesh K. Mahatma, A. L. Singh |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Directorate of Groundnut Research ICAR::Central Arid Zone Research Institute |
Published/ Complete Date: | 2022-06-25 |
Project Code: | Not Available |
Keywords: | Absorption · Phytic acid · Groundnut · Ion chromatography · Spectrophotometric method |
Publisher: | Springer |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | A study was made to expedite ion chromatography method using IonPac analytical column and self-regenerating anion suppressor for phytic acid determination in groundnut seeds and compared with a widely adopted spectrophotometric method based on enzymatic hydrolysis. The Ion Chromatography method equipped with AG11 guard and AS11 analytical columns in isocratic mode using 65 mM NaOH mobile phase at 1 mL min−1 fow rate showed a sharp peak for phytic acid with a retention time of 2.42±0.2 min. The peak area was plotted v/s concentration showed linearity with an R2 value of 0.997, detection limit of 0.028 mg L−1 and recovery of 98% as against R2 value of 0.988 and detection limit of 0.065 mg L−1 in the spectrophotometric method. The study demonstrates that Ion Chromatography method was more accurate with a better detection limit than spectrophotometry. Also, this method provides robust handling with lesser reagent requirements due to combined eluent generation and self-regenerating suppression. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Journal of Food Science and Technology |
NAAS Rating: | 8.7 |
Impact Factor: | 3.1 |
Volume No.: | Not Available |
Page Number: | Not Available |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | https://doi.org/10.1007/s13197-022-05527-9 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/73596 |
Appears in Collections: | CS-DGR-Publication |
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