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http://krishi.icar.gov.in/jspui/handle/123456789/74058| Title: | Expression of short hairpin RNA (shRNA) targeting AC2 gene of Mungbean yellow mosaic India virus (MYMIV) reduces the viral titre in soybean |
| Other Titles: | Not Available |
| Authors: | S.V. Ramesh M. Shivakumar Shelly Praveen |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | Indian Institute of Soybean Research Indian Agricultural Research Institute |
| Published/ Complete Date: | 2019-08-16 |
| Project Code: | DSR 1.24/12 |
| Keywords: | Begomovirus, MYMIV, RNAi, shRNAs,Soybean,Virus resistance |
| Publisher: | Not Available |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Mungbean yellow mosaic India virus (MYMIV) belonging to the family Geminiviridae and the genus Begomovirus is a severe pathogen of tropical legumes including soybean. The absence of genetically mapped loci conferring resistance together with the genetic diversity of begomoviruses infecting soybean warrants the utilization of RNA interference (RNAi) technology to develop virus resistance. However, viral suppressors of RNAi (VSRs) reduce the efectiveness of RNA silencing. Here, we report the efectiveness of Agrobacterium-mediated transient expression of shRNA, targeting a conserved region of AC2 ORF (a VSR) of MYMIV, in conferring virus resistance in soybean. Transient expression of shRNA showed progressive reduction of the viral titre estimated by the MYMIV-derived AC2 gene copy numbers from the initial inoculum by approximately 80-fold 20 days post-application. In addition, the newly emerging leaves exhibited symptom recovery. Thus, this study proves that AC2 of MYMIV is a potent target gene for obtaining RNAi-mediated virus resistance in soybean. Agroinfltration-based delivery of shRNA was an efcient means of gene silencing and could pave way for the development of transgenic virus-resistant soybean genotype |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | 3 Biotech |
| NAAS Rating: | 7.80 |
| Volume No.: | Not Available |
| Page Number: | Not Available |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | https://doi.org/10.1007/s13205-019-1865-7 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/74058 |
| Appears in Collections: | CS-DSBR-Publication |
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