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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/74985
Title: | Molecular mapping of a new recessive wheat leaf rust resistance gene originating from Triticum spelta |
Other Titles: | Not Available |
Authors: | V. Dinkar, S. K. Jha, N. Mallick, M. Niranjana, P. Agarwal, J. B. Sharma and Vinod |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Indian Agricultural Research Institute |
Published/ Complete Date: | 2020-12-17 |
Project Code: | Not Available |
Keywords: | Wheat, Triticum spelta, Puccinia triticina, SSR, SNP, Mapping |
Publisher: | Nature |
Citation: | Dinkar, V., Jha, S.K., Mallick, N., Niranjana, M., Agarwal, P. and Sharma, J.B., 2020. Molecular mapping of a new recessive wheat leaf rust resistance gene originating from Triticum spelta. Scientific reports, 10(1), pp.1-9.e |
Series/Report no.: | Not Available; |
Abstract/Description: | TSD276-2, a wheat genetic stock derived from the cross Agra Local/T. spelta 276 showed broad spectrum resistance against leaf rust pathogen. Genetic analysis was undertaken using F1, F2, F2:3 and BC1F1 generations derived from the cross TSD276-2/Agra Local. The results revealed a single recessive gene for leaf rust resistance, tentatively named as LrTs276-2, in TSD276-2. Molecular mapping of leaf rust resistance gene LrTs276-2 in TSD276-2 was done using SNP-based PCR and SSR markers. For Bulked Segregant Analysis (BSA), two bulks viz. resistant bulk and susceptible bulk, and the parents TSD276-2 and Agra Local were genotyped for SNPs using AFFYMETRIX 35K Wheat Breeders’ AXIOM array. T. spelta 276 was also genotyped and used as a check. BSA indicated that the gene for leaf rust resistance in TSD276-2 is located on chromosome arm 1DS. Putatively linked SNPs on chromosome arm 1DS were converted into PCR-based markers. Polymorphic SSR markers on chromosome arm 1DS were also identifed. Final linkage map was constructed using one SNP-based PCR and three SSR markers. The rust reaction and chromosomal location suggest that LrTs276-2 is a new leaf rust resistance gene which may be useful in broadening the genetic base of leaf rust resistance in wheat. |
Description: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | NAHEP |
Language: | English |
Name of Journal: | Scientific Reports |
Journal Type: | Peer-reviewed |
NAAS Rating: | 10.38 |
Impact Factor: | 4.38 |
Volume No.: | 10 |
Page Number: | 1-9 |
Name of the Division/Regional Station: | Division of Genetics |
Source, DOI or any other URL: | https://doi.org/10.1038/s41598-020-78679-3 |
URI: | https://doi.org/10.1038/s41598-020-78679-3 http://krishi.icar.gov.in/jspui/handle/123456789/74985 |
Appears in Collections: | AEdu-NAARM-Publication |
Files in This Item:
File | Description | Size | Format | |
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Molecular mapping of a new recessive wheat leaf rust resistance gene originating from Triticum spelta.pdf | 1.71 MB | Adobe PDF | View/Open | |
suppli 1.pdf | 669.81 kB | Adobe PDF | View/Open | |
Suppli 2.pdf | 546.92 kB | Adobe PDF | View/Open |
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