Nutritional requirements for mycelial growth and cultivation of Flammulina velutipes

Abstract

Physiological studies on Flammulina velutipes revealed that out of fourteen solid media tested, Glucose asparagine agar medium supported the maximum radial mycelial growth (90 mm) followed by Malt extract agar medium (87 mm) and Rice extract agar medium (84 mm). Glucose asparagine was the best (11 mg/ml) among all the nine liquid media tested. Optimum temperature and pH for mycelial growth were 25C and 8.0, respectively. Among thirteen carbon and nine nitrogen sources tested, the best growth was supported by sucrose and asparagine, respectively. All the four trace elements supported good growth of the fungus, however, 1 ppm FeSo4, 2 ppm ZnSO4 and MnSo4 and 10 ppm Ammonium molybdate proved superior as compared to higher or lower concentrations of the same trace element tried. Among the four growth regulators, gibberellic acid supported the maximum (10.2 mg/ml) mycelial growth at 100 ppm concentration. Pasture grass supported the fastest growth among all the eight substrates tested. In cultivation, trial biological efficiency of thirty per cent was obtained on saw dust of broad tree leaves.