Species Composition and Distribution of the Vector Aphids of PVY and PLRV in India

Abstract

Aphid transmitted viruses are the most significant constraint for quality seed potato production. Potato virus Y and Potato leaf roll virus are the most important aphid transmitted viruses in potato, which can be transmitted by a large number of aphid species. In order to determine the species composition of aphids infesting potato crops and to determine their viruliferous nature, aphid samples were collected from all the potatoes growing zones in India. Fourteen distinct aphid species were identified from 541 samples using morphological and molecular characterization. The identified speciesincludeAphiscraccivora,A.gossypii,A.fabae,A.spiraecola,Aulacorthumsolani, Macrosiphumeuphorbiae,Rhopalosiphumrufiabdominale,andMyzuspersicaeamong the colonizing species and A. nerii, R. padi, R. maidis, Hyadaphis coriandri, Brevicoryne brassicae, andLipaphis erysimi among the non-colonizing species. M. persicae and A. gossypii were the most abundant and most widespread species on potato in the country. Based on whole body testing of aphid samples through RT-PCR, M. persicae, A. gossypii, A. solani, andM. euphorbiae were found positive for PVY while M. persicae and A. gossypii tested positive for PLRV, which could potentially transmit the respective viruses in potato crops. The current study revealed that aphid species other than M.persicae could be important in determining the virus incidence in seed crops in the country. Therefore, management decisions should include such information to maintain the health standard of seed stocks.