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http://krishi.icar.gov.in/jspui/handle/123456789/75200
Title: | Establishment of axenic cultures in Pomegranate cultivars Bhagwa and Super Bhagwa |
Other Titles: | Not Available |
Authors: | K Antony Prajwala BVK Bhagavan K Ravindra Kumar P Subbaramamma K Prabhakara Rao* T Gouri Sankar |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Central Tobacco Research Institute |
Published/ Complete Date: | 2022-03-29 |
Project Code: | Not Available |
Keywords: | Pre-treatment Surface sterilization Bhagwa Super Bhagwa |
Publisher: | Not Available |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Success of a commercially viable protocol for mass multiplication of plants using tissue culture begins with effective elimination of microbial contamination by pre-treatment and surface sterilization methods. In the present study, nodal explants of pomegranate cv. Bhagwa and Super Bhagwa were established using different durations and concentrations of anti-microbial agents followed by inoculation in the culture media for initiation. Among different treatments tested, it was observed that in both the cultivars explants pre-treated with Carbendazim 0.2% + (Metalaxyl-M + Mancozeb) 0.2% + 8 HQ 200ppm for 1 h followed by surface sterilization in laminar airflow chamber with Mercuric chloride (HgCl2)@ 0.1% for 6 min. was proved to be effective method in culture establishment, with maximum survival percentage (57.13% and 67.41% respectively). Among the cultivars Bhagwa showed the maximum survival percentage (33.67% and 36.43%, respectively) when treated with Carbendazim 0.2% + (Metalaxyl-M + Mancozeb) 0.2% + 8 HQ 200ppm for 1 h followed by surface sterilization with Mercuric chloride (HgCl2) @ 0.1% for 6 min with minimum mortality of the explants. |
Description: | Not Available |
ISSN: | 2349-8242 |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | The Pharma Innovation Journal |
NAAS Rating: | 5.23 |
Volume No.: | 11(4) |
Page Number: | 35-40 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/75200 |
Appears in Collections: | CS-CTRI-Publication |
Files in This Item:
File | Description | Size | Format | |
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prabhakar-2.pdf | 724.34 kB | Adobe PDF | View/Open |
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