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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/76849
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Varughese HS | en_US |
dc.contributor.author | Chandrahasa C | en_US |
dc.contributor.author | Kavitha GM | en_US |
dc.contributor.author | Akhila DS | en_US |
dc.contributor.author | Gopinath S | en_US |
dc.contributor.author | Govindaiah K | en_US |
dc.contributor.author | Chandan T | en_US |
dc.contributor.author | Rao VCP | en_US |
dc.contributor.author | Hosamani M | en_US |
dc.contributor.author | Ramakrishnaiah S | en_US |
dc.contributor.author | Doddamane R | en_US |
dc.contributor.author | Hemadri D | en_US |
dc.contributor.author | Isloor S | en_US |
dc.date.accessioned | 2023-04-13T06:33:52Z | - |
dc.date.available | 2023-04-13T06:33:52Z | - |
dc.date.issued | 2022-12-12 | - |
dc.identifier.citation | Varughese HS, Chandrahasa C, Kavitha GM, Akhila DS, Gopinath S, Govindaiah K, Chandan T, Rao VCP, Hosamani M, Ramakrishnaiah S, Doddamane R, Hemadri D and Isloor S. (2022). Development of recombinant partial nucleoprotein of rabies virus as the potential antigen for developing in house immunodiagnostics. The Pharma Innovation Journal, 12 516-519 | en_US |
dc.identifier.issn | 2277-7695 | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/76849 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Rabies viral nucleoprotein is proven to be antigenic and immunogenic in nature. Monoclonal antibody binding characterises the Rabies viral nucleoproteins immune reactivity. However, studies on reactivity of nucleoproteins to polyclonal antibodies are limited. In this study, a partial Rabies viral nucleoprotein gene of 516 bp was cloned into a pET 32a vector, and prokaryotic expression was induced to obtain a protein of 24.1 kDa. The protein obtained was further characterised by Western blot and indirect ELISA. Our research discovered Rabies nucleoprotein immune reactivity in polyclonal sera and suspected that the epitopes are conformational in nature. This study adds to the limited number of polyclonal sera based studies on Rabies nucleoprotein. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | The Pharma Innovation | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Rabies | en_US |
dc.subject | nucleoprotein | en_US |
dc.subject | cloning | en_US |
dc.subject | in house immunodiagnostics | en_US |
dc.title | Development of recombinant partial nucleoprotein of rabies virus as the potential antigen for developing in house immunodiagnostics | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | The Pharma Innovation Journal | en_US |
dc.publication.volumeno | 12 | en_US |
dc.publication.pagenumber | 516-519 | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | https://www.thepharmajournal.com/archives/2023/vol12issue1/PartF/11-12-644-103.pdf | en_US |
dc.publication.authorAffiliation | Department of Veterinary Microbiology, Veterinary College, KVAFSU, Bengaluru, Karnataka, India | en_US |
dc.publication.authorAffiliation | Nitte University Centre for Science Education and Research, Deralakatte, Mangaluru, Karnataka, India | en_US |
dc.publication.authorAffiliation | ICAR Indian Veterinary Research Institute, Bengaluru, Karnataka, India | en_US |
dc.publication.authorAffiliation | ICAR National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka, India | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.journaltype | Peer reviewed journal | en_US |
dc.publication.naasrating | 5.23 | en_US |
dc.publication.impactfactor | Not listed in Impact Factor List | en_US |
Appears in Collections: | AS-NIVEDI-Publication |
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