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http://krishi.icar.gov.in/jspui/handle/123456789/76914
Title: | Effect of Experimental Foot and Mouth Disease Virus Infection on the Activation and Polarization of Bovine Monocyte-Derived Macrophages |
Other Titles: | Not Available |
Authors: | 38. Sebastian, Renjith and Sravanthi, Mannem and Vijayapillai, Umapathi and Krishnaswamy, Narayanan and Mahadappa, Priyanka and Dechamma, Hosur J. and Basagoudanavar, Suresh H. and Sanyal, Aniket and Kondabattula, Ganesh and Reddy, G.R |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Indian Veterinary Research Institute Bangalore |
Published/ Complete Date: | 2022-08-01 |
Project Code: | Not Available |
Keywords: | FMDV, Bovine, Monocyte-derived macrophage, Macrophage polarization, Innate immune response |
Publisher: | SSRN |
Citation: | Sebastian, Renjith and Sravanthi, Mannem and Vijayapillai, ,. Umapathi and Krishnaswamy, Narayanan and Mahadappa, Priyanka and Dechamma, Hosur J. and Basagoudanavar, Suresh H. and Sanyal, Aniket and Kondabattula, Ganesh and Reddy, G.R, Effect of Experimental Foot and Mouth Disease Virus Infection on the Activation and Polarization of Bovine Monocyte-Derived Macrophages . Available at SSRN: https://ssrn.com/abstract=4170666 or http://dx.doi.org/10.2139/ssrn.4170666 |
Series/Report no.: | Not Available; |
Abstract/Description: | Foot and mouth disease virus (FMDV) causes an economically devastating, contagious viral disease in cloven-hoofed animals. Macrophages are potent mediators of innate immune responses against viruses. The macrophage-FMDV interaction is not studied in detail, especially the innate immune responses to the virus. Here, we investigated the effects of experimental infection of FMDV on bovine monocyte-derived macrophages (MDM). The detection of negative-strand FMDV RNA by strand specific RT-PCR and demonstration of viral antigen by immunofluorescence indicated the replication of FMDV RNA and synthesis of viral proteins, respectively in the infected MDM. However, replication kinetic experiments revealed that the copy number of FMDV transcripts and virus titration peaked at 12 hpi with subsequent reduction, indicating non-progressive replication. FMDV infection upregulated the expression of RIG1 and MDA5 in MDM, suggests that the FMDV RNA are sensed by RLRs. In addition, nearly 75% of the infected MDM differentiated into M1 phenotype, characterized by the increased expression of M1-specific markers; CD80, iNOS and proinflammatory cytokines such as TNFα and IL12. The infection induced the expression of Type I IFNs, which coincided with the decline in the viral RNA and progeny virus after 12 hpi. In addition, infected MDM abundantly expressed ISGs such as PKR, OAS1, Mx1 and viperin against FMDV infection at 12 hpi. In conclusion, FMDV underwent a non-progressive replication in the bovine MDM that might be due to induction of M1 polarization and upregulation of antiviral genes. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | ICAR-IVRI |
Language: | English |
Name of Journal: | ONLINE SSRN |
Journal Type: | Not Included in NAAS Journal |
NAAS Rating: | 7 |
Impact Factor: | 1 |
Volume No.: | Not Available |
Page Number: | Not Available |
Name of the Division/Regional Station: | ICAR::Indian Veterinary Research Institute Bangalore |
Source, DOI or any other URL: | dx.doi.org/10.2139/ssrn.4170666 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/76914 |
Appears in Collections: | AS-IVRI-Publication |
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