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http://krishi.icar.gov.in/jspui/handle/123456789/77120| Title: | Detection of replication competent adenovirus upon serial passaging of recombinant adenovirus expressing FMDV capsid proteins. |
| Other Titles: | Not Available |
| Authors: | R Kumar H B Suresh B P Sreenivasa |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Indian Veterinary Research Institute |
| Published/ Complete Date: | 2015-05-01 |
| Project Code: | Not Available |
| Keywords: | HEK-293 Human adenovirus type 5 FMD virus |
| Publisher: | Biologicals |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Replication deficient human adenovirus type 5 (hAd5) is an important gene delivery vehicle and has been used in various fields of biomedical sciences such as gene therapy, cancer therapy and vaccination. Inspite of its various useful features, emergence of replication competent adenovirus (RCA) in recombinant virus stocks is a great concern. In the present study, recombinant adenovirus expressing foot-and-mouth disease virus serotype-O capsid proteins was propagated in HEK-293 cells and purified by CsCl density gradient ultracentrifugation. The virus was serially passaged in HEK-293 cells and at passage level (P) 2–4, 6, 8 and 12, tested for the presence of RCA. A vector dose of 2 × 108 and 1 × 1010 TCID50 of the virus was used in cell line bioassay and PCR, respectively. Our results demonstrated that the PCR is more sensitive and rapid technique for the detection of RCA in recombinant adenovirus stocks as early as at P3, whereas the bioassay detected the RCA at P8. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Biologicals |
| Journal Type: | Included NAAS journal list |
| NAAS Rating: | 7.76 |
| Impact Factor: | 1.20 |
| Volume No.: | 43 (3) |
| Page Number: | 209-212 |
| Name of the Division/Regional Station: | ICAR::Indian Veterinary Research Institute bengaluru |
| Source, DOI or any other URL: | https://doi.org/10.1016/j.biologicals.2015.02.002 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/77120 |
| Appears in Collections: | AS-IVRI-Publication |
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