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http://krishi.icar.gov.in/jspui/handle/123456789/77179| Title: | Expression of bovine (Bos indicus) interleukin-18 in Escherichia coli and its biological activity |
| Other Titles: | Not Available |
| Authors: | K Siva Reddy Muralidhar Rao Dowlathabad Dechamma H. J Suryanarayana V. V.S. G.R. Reddy |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR- Indian veterinary research institute |
| Published/ Complete Date: | 2010-08-27 |
| Project Code: | Not Available |
| Keywords: | Bovine interlukin-18 E. coli |
| Publisher: | Wiley Online Library |
| Citation: | Reddy KS, Rao DM, Dechamma HJ, Suryanarayana VV, Reddy GR. Expression of bovine (Bos indicus) interleukin-18 in Escherichia coli and its biological activity. Microbiol Immunol. 2010 Sep;54(9):564-7. doi: 10.1111/j.1348-0421.2010.00241.x. PMID: 20840156. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | IL-18 modulates immune functions by inducing IFN-γ production and promoting Th1 immune responses. In the present study, we amplified and cloned the sequence (582 bp) encoding full-length bovine IL-18 from PBMC stimulated with PHA. The nucleotide and the deduced amino acid sequence of Bos indicus IL-18 showed an identity of 86-98% compared with IL-18 sequences of other ruminants. The insert was subcloned into a pET 32a vector and expressed in Escherichia coli as a fusion protein and the matured protein was obtained by caspase I treatment. The specificity of these proteins was confirmed by western blotting. The biological activity of the purified protein was analyzed by its ability to induce IFN-γ production in PBMC measured by ELISA and qPCR. |
| Description: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Microbiology and Immunology |
| Journal Type: | Included as NAAS JOURNAL |
| NAAS Rating: | 7.43 |
| Impact Factor: | 1.428 |
| Volume No.: | 54 |
| Page Number: | 564–567 |
| Name of the Division/Regional Station: | IVRI, Regional station Bangalore |
| Source, DOI or any other URL: | doi.org/10.1111/j.1348-0421.2010.00241.x |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/77179 |
| Appears in Collections: | AS-IVRI-Publication |
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