KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/77182
Title: | The plasmid constructs producing shRNA corresponding to the conserved 3D polymerase of Foot and Mouth Disease virus protects guinea pigs against challenge virus |
Other Titles: | Not Available |
Authors: | Dechamma Hosur Joyappa Sarika Sasi Kumar C. Ashok Golla Rama Reddy Veluvarthy V. S. Suryanarayana |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR- Indian veterinary research institute |
Published/ Complete Date: | 2008-09-23 |
Project Code: | Not Available |
Keywords: | Foot-and-mouth disease virus ShRNA RNAi |
Publisher: | Springer |
Citation: | Joyappa DH, Sasi S, Ashok KC, Reddy GR, Suryanarayana VV. The plasmid constructs producing shRNA corresponding to the conserved 3D polymerase of Foot and Mouth Disease virus protects guinea pigs against challenge virus. Vet Res Commun. 2009 Mar;33(3):263-71. doi: 10.1007/s11259-008-9174-3. Epub 2008 Sep 23. PMID: 18810649. |
Series/Report no.: | Not Available; |
Abstract/Description: | RNA interference (RNAi) has been used as an effective antiviral strategy for its specific silencing of viral gene expression in mammalian cells. In this study, shRNA targeting two regions of Foot and Mouth Disease Virus (FMDV) i.e. 3D and 5′UTR which are very essential in virus replication were evaluated. The constructs were made using h7K RNA polymerase III promoter. We investigated in vivo inhibitory effect of shRNA on FMDV replication in BHK-21 cells and guinea pigs. The results showed that transfection of 3D shRNA could reduce virus growth by three folds when cells were challenged with 102 TCID50 of FMDV. Pretreated guinea pigs with 3DshRNA were protected 80% with 103 GPID50 of FMDV. As a first report in guinea pigs which are recognized animal model for FMD vaccine potency testing, the study suggests that shRNA could be a viable therapeutic approach to control severity of FMD infection and spread. |
Description: | Not Available |
ISSN: | 1573-7446 |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Veterinary Research Communications |
Journal Type: | Included as NAAS JOURNAL |
NAAS Rating: | 7.29 |
Impact Factor: | 2.459 |
Volume No.: | 33 |
Page Number: | 263–271 |
Name of the Division/Regional Station: | IVRI, Regional station Bangalore |
Source, DOI or any other URL: | DOI: 10.1007/s11259-008-9174-3 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/77182 |
Appears in Collections: | AS-IVRI-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.