KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/77192| Title: | A new blocking ELISA for detection of foot-and-mouth disease non-structural protein (NSP) antibodies in a broad host range |
| Other Titles: | Not Available |
| Authors: | M Hosamani S Gopinath B P Sreenivasa S Behera S H Basagoudanavar A Boora D P Bora P Deka V Bhanuprakash R K Singh A Sanyal K Weerdmeester A Dekker |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Indian Veterinary Research Institute |
| Published/ Complete Date: | 2022-09-12 |
| Project Code: | Not Available |
| Keywords: | blocking ELISA Foot-and-mouth disease Monoclonal antibody |
| Publisher: | Applied Microbiology and Biotechnology |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Large-scale monitoring of foot-and-mouth disease (FMD) in livestock is imperative in an FMD control program. Detection of antibodies against non-structural proteins (NSP) of FMD virus (FMDV) is one of the best tools to estimate the prevalence of past infection; availability of such a well-validated test is therefore essential. Using a FMDV 3B protein-specific monoclonal antibody, we have developed a new NSP antibody blocking ELISA (10H9 bELISA) and validated it on large panels of sera from different susceptible species. The diagnostic sensitivity of the ELISA was 95% with a specificity of 98%, similar to the values found using a commercial kit (PrioCHECK FMD NS test). The 10H9 bELISA can be used in a broad range of FMD susceptible species making it a very useful tool in monitoring the foot-and-mouth disease control programs by detection of virus circulation in the vaccinated populations. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Applied Microbiology and Biotechnology |
| Journal Type: | Included NAAS journal list |
| NAAS Rating: | 11.56 |
| Impact Factor: | 5.56 |
| Volume No.: | 106(19-20) |
| Page Number: | 6745-6757 |
| Name of the Division/Regional Station: | ICAR::Indian Veterinary Research Institute Bengaluru |
| Source, DOI or any other URL: | 10.1007/s00253-022-12151-2 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/77192 |
| Appears in Collections: | AS-IVRI-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.