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http://krishi.icar.gov.in/jspui/handle/123456789/77201
Title: | Evaluation of a recombinant major envelope protein (F1L) based indirect- ELISA for sero-diagnosis of orf in sheep and goats. |
Authors: | Yogisharadhya R, Kumar A, Bhanuprakash V, Shivachandra SB |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR:Indian Veterinary Research Institute |
Published/ Complete Date: | 2018-08-24 |
Keywords: | Immunodiagnostic assay; Indirect-ELISA; Orf virus; Recombinant F1L protein; Small ruminants. |
Publisher: | Elsevier |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Orf or contagious ecthyma, is a highly contagious transboundary disease of sheep and goats. For sero-diagnosis of orf, recombinant antigen based assays are considered as alternatives to conventional approaches such as serum neutralization test (SNT) and counter-immuno-electrophoresis (CIE). A major envelope protein of orf virus (ORFV), F1L, is highly immunogenic and is a candidate for use in these assays. In this study, the F1L gene of the ORFV-59/05 strain encoding a recombinant mature F1L protein (1M-D302 aa) with a C- terminal truncation, was produced as a fusion protein (∼50 kDa) in Escherichia coli. The immunogenic potential of purified rF1L was confirmed by detecting specific anti-F1L antibody responses in sera collected from immunized rabbits and guinea pigs using ELISA and SNT. An indirect-ELISA based on rF1L was developed and optimized. In comparison to SNT by ROC analysis in the detection of ORFV specific antibodies, this new assay exhibited a diagnostic specificity of 94.04% and 92.53% with sheep and goat sera, respectively, while the sensitivity was 89.22% and 94.25%, for sheep and goat sera. No cross reactivity was noted with sera collected from small ruminants infected with other transboundary diseases (goatpox, sheeppox, peste des petits ruminants, foot-and-mouth disease and bluetongue). Furthermore, the rF1L-ELISA applied to screen the vaccinated/challenged goat sera resulted in better detection (30%) than by SNT (28%) in spite of lower levels of antibodies which could be due to predominant cell mediated immune response in vaccinated animals. This study highlighted the potential utility of rF1L protein as a safe and novel diagnostic reagent in comparison to live virus antigen, in the development of sero-diagnostic assay for surveillance of ORFV infection in sheep and goats. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Journal of Virological Methods |
Journal Type: | Included in NAAS Journal list |
NAAS Rating: | 8.62 |
Impact Factor: | 2.623 |
Volume No.: | 261 |
Page Number: | 112-120 |
Name of the Division/Regional Station: | ICAR-IVRI Campus, Hebbal, Bengaluru 560024, Karnataka |
Source, DOI or any other URL: | 10.1016/j.jviromet.2018.08.015 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/77201 |
Appears in Collections: | AS-IVRI-Publication |
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