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http://krishi.icar.gov.in/jspui/handle/123456789/77227
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shivachandra SB, Yogisharadhya R, Ahuja A, Bhanuprakash V. | en_US |
dc.date.accessioned | 2023-05-12T10:13:29Z | - |
dc.date.available | 2023-05-12T10:13:29Z | - |
dc.date.issued | 2012-12-01 | - |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/77227 | - |
dc.description.abstract | Pasteurella multocida serogroup B:2, a causative agent of haemorrhagic secpticaemia (HS) in cattle and buffalo especially in tropical regions of Asia and African countries, is known to possess a type IV fimbriae (pili) as one of the virulent factors. In the present study, ptfA gene encoding for type IV fimbrial subunit of P. multocida serogroup B:2 (strain p52), an Indian HS vaccine strain, has been cloned and over-expressed in recombinant Escherichia coli. The recombinant type IV fimbrial subunit protein (∼31 kDa) including N-terminus histidine tag was purified under denaturing condition and confirmed by western blotting. A homology model of HS causing P. multocida serogroup B:2 fimbrial subunit has also been discussed. The study indicated the potential possibilities to use the recombinant fimbrial protein in developing HS subunit vaccine along with suitable adjuvant. | en_US |
dc.language.iso | English | en_US |
dc.publisher | Elsevier | en_US |
dc.subject | P multocida, E Coli, Type IV fimbriae, Expression, Purification | en_US |
dc.title | Expression and purification of recombinant type IV fimbrial subunit protein of Pasteurella multocida serogroup B:2 in Escherichia coli. | en_US |
dc.type | Research Paper | en_US |
dc.publication.journalname | Research In Veterinary Science | en_US |
dc.publication.volumeno | 93(3) | en_US |
dc.publication.pagenumber | 1128-1131 | en_US |
dc.publication.divisionUnit | ICAR-IVRI Campus, Hebbal, Bengaluru 560024, Karnataka | en_US |
dc.publication.sourceUrl | 10.1016/j.rvsc.2012.02.010 | en_US |
dc.publication.authorAffiliation | ICAR:Indian Veterinary Research Institute | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.journaltype | Included in NAAS Journal list | en_US |
dc.publication.naasrating | 8.55 | en_US |
dc.publication.impactfactor | 2.554 | en_US |
Appears in Collections: | AS-IVRI-Publication |
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