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http://krishi.icar.gov.in/jspui/handle/123456789/77240
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Balamurugan V, Sen A, Venkatesan G, Yadav V, Bhanot V, Bhanuprakash V, Singh RK. | en_US |
dc.date.accessioned | 2023-05-12T10:28:35Z | - |
dc.date.available | 2023-05-12T10:28:35Z | - |
dc.date.issued | 2012-02-01 | - |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/77240 | - |
dc.description.abstract | A sensitive and rapid single step real time (rt) RT-PCR was standardized using one-step Brilliant SYBR Green kit® for detection and semi-quantitation of peste des petitis ruminants virus (PPRV) using the virus RNA and matrix (M) protein gene-specific primers and compared with established conventional RT-PCR and TaqMan RT-PCR. The assay amplifies a 124 bp fragment of the PPRV M gene with T(m) of 78.28 to 78.50. The assay was linear within a range of 50 ng to 0.5 fg total virus RNA with a detection limit (sensitivity) of 0.5 fg. Based on the serial dilution of the live-attenuated PPR vaccine virus, the detection limit was ~0.0001 cell culture infectious dose 50% units (TCID(50)). Additionally, swab materials spiked with known titre of vaccine virus were equally well detected in the assay. The standardized rt RT-PCR was easily employed for the detection of PPRV nucleic acid directly in the field and experimental clinical samples. The assay detected the PPRV nucleic acid as early as 3 day post infection (dpi) and up to 20 dpi in swab materials from the experimental samples. The assay was rapid and more sensitive than TaqMan and conventional RT-PCR in the detection of PPRV nucleic acid from the PPR suspected clinical samples of sheep and goats. Therefore, the established, simplified SYBR green rt RT-PCR is an alternative test to the already existing various diagnostic assays and could be useful for rapid clinical diagnosis with advantage in reducing risk of contamination. | en_US |
dc.language.iso | English | en_US |
dc.publisher | Elsevier | en_US |
dc.subject | SYBR Green, RT PCR, Detection, PPRV, Clinical samples | en_US |
dc.title | A rapid and sensitive one step-SYBR green based semi quantitative real time RT-PCR for the detection of peste des petits ruminants virus in the clinical samples. | en_US |
dc.type | Research Paper | en_US |
dc.publication.journalname | Virologica Sinica | en_US |
dc.publication.volumeno | 27(1) | en_US |
dc.publication.pagenumber | 1-9 | en_US |
dc.publication.divisionUnit | ICAR-IVRI Campus, Hebbal, Bengaluru 560024, Karnataka | en_US |
dc.publication.sourceUrl | 10.1007/s12250-012-3219-z | en_US |
dc.publication.authorAffiliation | ICAR:Indian Veterinary Research Institute | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.journaltype | Included in NAAS Journal list | en_US |
dc.publication.naasrating | 12.95 | en_US |
dc.publication.impactfactor | 6.947 | en_US |
Appears in Collections: | AS-IVRI-Publication |
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