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http://krishi.icar.gov.in/jspui/handle/123456789/77451| Title: | Integrated analysis of long-noncoding RNA and circular RNA expression in Peste-des-Petits-Ruminants Virus (PPRV) infected marmoset B lymphocyte (B95a) cells |
| Authors: | Gunturu Narasimha Tanuj Owais Khan Waseem Akram Malla Kaushal Kishore Rajak S Chandrashekar Amit Kumar Sujoy K Dhara Praveen K Gupta Bishnu Prasad Mishra Triveni Dutt RaviKumar Gandham Basavaraj K Sajjanar |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Indian Veterinary Research Institute ICAR::National Bureau of Animal Genetic Resources |
| Published/ Complete Date: | 2022-09-30 |
| Project Code: | DBT/Wellcome Trust India Alliance |
| Keywords: | B-lymphocytes; Non-coding RNA; PPRV; Virus-host interactions |
| Publisher: | Elsevier |
| Citation: | Tanuj GN, Khan O, Malla WA, Rajak KK, Chandrashekar S, Kumar A, Dhara SK, Gupta PK, Mishra BP, Dutt T, Gandham R, Sajjanar BK. Integrated analysis of long-noncoding RNA and circular RNA expression in Peste-des-Petits-Ruminants Virus (PPRV) infected marmoset B lymphocyte (B95a) cells. Microb Pathog. 2022 Sep;170:105702. doi: 10.1016/j.micpath.2022.105702 |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Peste-des-Petits-Ruminants (PPR) or goat plague is an important viral disease of sheep and goats caused by the small ruminant morbilli virus or PPR virus (PPRV). Long non coding RNAs (lncRNA) and circular RNAs (circRNA) play a pivotal role in several biological processes including regulation of virus-host interactions. The present study explored the expression of lncRNA, circRNA and their functions in PPRV infected B-lymphocyte (B95a) cells. The results revealed a total of 4531 lncRNA and 2348 circRNA expression in both mock and PPRV infected samples. Analysis of differentially expressed (DE) RNA identified 123 DE-lncRNA and 39 DE-circRNA as significantly dysregulated. Functional analysis of cis-target genes of DE-lncRNA indicated activation of TCF dependent WNT signaling and PKN1 stimulated transcription process. Interactions (sponging) of microRNA (miRNA) revealed 344 DE-lncRNA-miRNA and 93 DE-circRNA-miRNA pairs. The competing endogenous RNA (ceRNA) network of lncRNA/circRNA-miRNA-mRNA in PPRV infected B95a cells was represented by 69 ceRNA pairs. We validated the DE-circRNA by targeted amplification and sequencing of back spliced junctions (BSJs). The present study revealed a profile of lncRNA, circRNA and their potential ceRNA network in PPRV infection. The results provide insight for better understanding of PPRV-host interactions. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | DBT/Wellcome Trust India Alliance Grant no: IA/E/17/1/503689 awarded to Basavaraj Sajjanar. |
| Language: | English |
| Name of Journal: | Microbial Pathogenesis |
| Journal Type: | Included NAAS journal list |
| NAAS Rating: | 9.85 |
| Impact Factor: | 3.85 |
| Volume No.: | 170 |
| Page Number: | 105702 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | 10.1016/j.micpath.2022.105702 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/77451 |
| Appears in Collections: | AS-IVRI-Publication |
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