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http://krishi.icar.gov.in/jspui/handle/123456789/77702| Title: | Direct detection of bovine herpesvirus-1 DNA from cell culture fluids using polymerase chain reaction |
| Authors: | B P Sreenivasa T J Rasool C Natarajan |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Indian Veterinary Research Institute |
| Published/ Complete Date: | 1996-11-01 |
| Project Code: | Not Available |
| Keywords: | polymerase chain reaction bovine herpesvirus 1 genomic DNA |
| Publisher: | Indian Journal of Experimental Biology |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | A pair of oligomers of 20 and 23 bp were designed for amplifying a 381 bp sequence from glycoprotein IV gene of bovine herpesvirus 1. The primer pairs were used for amplifying genomic DNA of BHV-1 directly from cell culture fluids under different experimental conditions such as, untreated cell culture fluid, thermal denaturation and proteinase K treatment in presence of detergent. The results reveal that direct thermal denaturation of cell culture fluid is sufficient to detect the virus by polymerase chain reaction. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Indian Journal of Experimental Biology |
| Journal Type: | Included NAAS journal list |
| NAAS Rating: | 6.94 |
| Impact Factor: | 0.94 |
| Volume No.: | 34 |
| Page Number: | 1169-1171 |
| Name of the Division/Regional Station: | ICAR::Indian Veterinary Research Institute, Bengaluru |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/77702 |
| Appears in Collections: | AS-IVRI-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| BHV-1 Direct detection.pdf | 7.64 MB | Adobe PDF | View/Open |
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