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http://krishi.icar.gov.in/jspui/handle/123456789/77846
Title: | Differentiation of sheep pox and goat poxviruses by sequence analysis and PCR-RFLP of P32 gene |
Other Titles: | Not Available |
Authors: | Hosamani M, Mondal B, Tembhurne PA, Bandyopadhyay SK, Singh RK, Rasool TJ |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Indian Veterinary Research Institute |
Published/ Complete Date: | 2004-08-01 |
Project Code: | Not Available |
Keywords: | sheep pox virus goat poxvirus Sequence analysis |
Publisher: | Springer link |
Citation: | Differentiation of sheep pox and goat poxviruses by sequence analysis and PCR-RFLP of P32 gene |
Series/Report no.: | Not Available; |
Abstract/Description: | Sheep pox and Goat pox are highly contagious viral diseases of small ruminants. These diseases were earlier thought to be caused by a single species of virus, as they are serologically indistinguishable. P32, one of the major immunogenic genes of Capripoxvirus, was isolated and Sequenced from two Indian isolates of goat poxvirus (GPV) and a vaccine strain of sheep poxvirus (SPV). The sequences were compared with other P32 sequences of capripoxviruses available in the database. Sequence analysis revealed that sheep pox and goat poxviruses share 97.5 and 94.7% homology at nucleotide and amino acid level, respectively. A major difference between them is the presence of an additional aspartic acid at 55th position of P32 of sheep poxvirus that is absent in both goat poxvirus and lumpy skin disease virus. Further, six unique neutral nucleotide substitutions were observed at positions 77, 275, 403, 552, 867 and 964 in the sequence of goat poxvirus, which can be taken as GPV signature residues. Similar unique nucleotide signatures could be identified in SPV and LSDV sequences also. Phylogenetic analysis showed that members of the Capripoxvirus could be delineated into three distinct clusters of GPV, SPV and LSDV based on the P32 genomic sequence. Using this information, a PCR-RFLP method has been developed for unequivocal genomic differentiation of SPV and GPV. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Article |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Virus Genes |
Journal Type: | included NAAS journal list |
NAAS Rating: | 8.33 |
Impact Factor: | 2.198 |
Volume No.: | 29(1) |
Page Number: | 73-80 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | https://doi.org/10.1023/b:viru.0000032790.16751.13 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/77846 |
Appears in Collections: | AS-IVRI-Publication |
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