Assessment of the relationship between serum neutralizing antibody titre and liquid phase blocking ELISA titre in immune response to FMDV Vaccine

Abstract

Traditionally, quality assessment of FMD vaccine is carried out by live FMD virus challenge of vaccinated cattle. Replacing the use of animals for quality assessment with in vitro tests like serum neutralization test (SNT) or liquid phase blocking ELISA (LPBE), requires the establishment of logistic regression models from series of challenge experiments. Before establishing a logistic regression model, we did a preliminary investigation on the relationship between SNT and LPBE titre, to be used as cut-off titre for classifying cattle as protected or unprotected.