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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/77915
Title: | Development of a novel multiplex PCR for detection of Brucella, Leptospira and Bovine herpesvirus-1 |
Other Titles: | Not Available |
Authors: | S. K. Bhure S. Chandan R. G. Amachawadi S. S. Patil Rajeswari Shome N. L. Gangadhar M R Gajendragad K Prabhudas |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::National Institute of Veterinary Epidemiology and Disease Informatics |
Published/ Complete Date: | 2012-01-01 |
Project Code: | Not Available |
Keywords: | Bovine Herpesvirus-1 Brucella Leptospira Infectious bovine rhenotracheitis Multiplex PCR |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | A novel multiplex PCR (mPCR) was developed for detection of Brucella, Leptospira and bovine herpesvirus-1 (BoHV-1) targeting conserved regions of BCSP31, LipL32 and gB genes, respectively. No template controls were also run with each test. Electrophoresis of mPCR products showed bands specific to genus Brucella, Leptospira and BHV- 1, respectively. The assay detected as low as 15 pg of each template. The mPCR showed specific bands with 4 Brucella species, 14 pathogenic Leptospira serovars, BoHV-1. In addition, 10 (out of 135 bull semen samples) and 5 (out of 17 bovine blood samples) showed a ~640bp amplicon specific to brucellosis. Five human urine samples were tested; 3 found positive for Leptospira and other 2 were positive for both Leptospira and Brucella. The assay is accurate, specific and cost effective tool for detection of Brucella, Leptospira and bovine herpesvirus-1 and will also be a valuable tool for the diagnoses of co-infection. The assay could be a preferable detection methodology for routine screening and molecular epidemiology of brucellosis, leptospirosis and infectious bovine rhenotracheitis. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Indian Journal of Animal Sciences |
Journal Type: | NAAs Rated |
NAAS Rating: | 6.29 |
Impact Factor: | 0.29 |
Volume No.: | 82 (11) |
Page Number: | 1285–1289 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | https://epubs.icar.org.in/index.php/IJAnS/article/view/25127 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/77915 |
Appears in Collections: | AS-IVRI-Publication |
Files in This Item:
File | Description | Size | Format | |
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2012 Development of a novel multiplex PCR for detection of Brucella, Leptospiraand Bovine herpesvirus-1.pdf | 128.71 kB | Adobe PDF | View/Open |
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