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http://krishi.icar.gov.in/jspui/handle/123456789/77923
Title: | Effect of actin polymerization inhibitor during oocyte maturation on parthenogenetic embryo development and their ploidy in Capra hircus |
Other Titles: | Not Available |
Authors: | Ranjan R. Singh R. K. Yasotha T. Kumar Manish. Puri Gopal. Kumar Kuldeep Singh Renu Bhure Sanjeev Malakar D. Bhanja S. K. Sarkar M. Das B. C. Bag Sadhan. |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Indian Veterinary Research Institute |
Published/ Complete Date: | 2013-07-12 |
Project Code: | Not Available |
Keywords: | cytochalasin B |
Publisher: | Springer |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | This study was designed to observe the effect of cytochalasin B (CCB) concentrations on ploidy and early development of parthenogenetic embryos in a caprine species. Caprine oocytes were matured in the presence of different concentrations of CCB (5, 10, 15, and 20 μg/ml) and activated by 7% ethanol followed by incubation with 2 mM DMAP. For embryos fertilized in vitro, oocytes were matured in maturation medium without CCB. The cleavage rate and further embryo development were significantly higher (P < 0.05) when oocytes were treated in this way. The percentage of embryos showed higher diploid values in 15 μg/ml CCB (83.66 ± 1.13), followed by 20 (72.22 ± 1.22), 10 (68.57 ± 1.17), and 5 μg/ml (62.00 ± 2.48). These results indicate that CCB with a concentration of 15 μg/ml in maturation medium can be used for the production of diploid parthenogenetic embryos in the caprine species. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Biochemical Genetics |
Journal Type: | NAAS Rated |
NAAS Rating: | 7.543 |
Impact Factor: | 1.543 |
Volume No.: | 51(11-12) |
Page Number: | 944-53 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | 10.1007/s10528-013-9619-4. |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/77923 |
Appears in Collections: | AS-IVRI-Publication |
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