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http://krishi.icar.gov.in/jspui/handle/123456789/78084| Title: | Expression Profile of HSP Genes during Different Seasons in Goats (Capra hircus) |
| Other Titles: | Not Available |
| Authors: | Dangi S S Gupta M Maurya D Yadav V P Panda R P Singh G Mohan N H Bhure S K Das B C Bag S Mahapatra R K Sarkar M |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Indian Veterinary Research Institute |
| Published/ Complete Date: | 2012-04-26 |
| Project Code: | Not Available |
| Keywords: | HSP genes Goat Heat stress |
| Publisher: | Springer |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | The present study has demonstrated the expression of HSP60, HSP70, HSP90, and UBQ in peripheral blood mononuclear cells (PBMCs) during different seasons in three different age groups (Groups I, II, and III with age of 0-2, 2-5, and >5 years, respectively) of goats of tropical and temperate regions. Real-time polymerase chain reaction was applied to investigate mRNA expression of examined factors. Specificity of the desired products was documented using analysis of the melting temperature and high-resolution gel electrophoresis to verify that the transcripts are of the exact molecular size predicted. The mRNA expression of HSP60, HSP90, and UBQ was significantly higher (P < 0.05) in all age groups during peak summer season as compared with peak winter season in both tropical and temperate region goats. HSP70 mRNA expression was significantly higher (P < 0.05) during summer season as compared with winter season in tropical region goats. However, in the temperate region, in goats from all the three age groups studied, a non-significant difference of HSP70 expression between summer and winter seasons was noticed. In conclusion, results demonstrate that (1) HSP genes are expressed in caprine PBMCs and (2) higher expression of HSPs during thermal stress suggest possible involvement of them to ameliorate deleterious effect of thermal stress so as to maintain cellular integrity and homeostasis in goats. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Tropical Animal Health and Production |
| Journal Type: | NAAS Rated |
| NAAS Rating: | 7.893 |
| Impact Factor: | 1.893 |
| Volume No.: | 44(8) |
| Page Number: | 1905-12 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | 10.1007/s11250-012-0155-8. |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/78084 |
| Appears in Collections: | AS-IVRI-Publication |
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