KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/78086
Title: | Molecular cloning and expression of buffalo ESX-1 gene: A marker for x-chromosome bearing spermatozoa |
Other Titles: | Not Available |
Authors: | Vijay N. P. Harikrishna S.K. Ghosh S.K. Bhure |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Indian Veterinary Research Institute |
Published/ Complete Date: | 2015-08-01 |
Project Code: | Not Available |
Keywords: | ESX-1 Buffalo |
Publisher: | ARCC |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Extra-embryonic tissue-spermatogenesis homeobox gene 1 (ESX-1), an X-linked gene that encodes homeobox protein, expresses specifically in X-chromosome bearing spermatozoa. The antibodies against this recombinant protein will be useful to assess the efficiency of sex sorted semen in buffalo. Therefore, the present study has been undertaken to clone and express the ESX-1 gene and then to study the characteristics of the expressed protein. In this investigation, the cDNA of ESX-1 was cloned, sequenced and expressed in E. coli. Predicted amino acid sequence of ESX-1 had shown highest homology with Bos taurus (91.6%). The homeobox region had shown the identity of 98.4%, 70.5%, 70.5%, 69.4% and 69.4% respectively with Bos taurus, Homo sapiens, Pan troglodytes, Macaca mulatta and Mus musculus. Expressed protein was purified using Cobalt-Agarose affinity column. SDS-PAGE showed a product ~27kDa recombinant fusion protein. The purified product was confirmed by Western blot analysis using Ni-NTA anti hisitidine HRPase conjugate. The antibodies against Esx-1 protein can be used for evaluation of sex sorted semen. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Indian Journal of Animal Research |
Journal Type: | NAAS Rated |
NAAS Rating: | 6.427 |
Impact Factor: | 0.427 |
Volume No.: | 49(4) |
Page Number: | 442-447 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | 10.5958/0976-0555.2015.00071.0. |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/78086 |
Appears in Collections: | AS-IVRI-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.