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http://krishi.icar.gov.in/jspui/handle/123456789/8074
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Pushpa K, Madhu P, Venkatesh Bhat B, Balakrishna D | en_US |
dc.date.accessioned | 2018-10-25T03:56:31Z | - |
dc.date.available | 2018-10-25T03:56:31Z | - |
dc.date.issued | 2017-12-01 | - |
dc.identifier.citation | Pushpa, K., Madhu, P., Venkatesh Bhat, B. and Balakrishna, D. 2017. “Effect of hygromycin on explant selection in genetic transformation experiments of sorghum (Sorghum bicolor (L.) Moench)”, International Journal of Current Research, 9, (12), 62294-62296. | en_US |
dc.identifier.issn | 0975-833X | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/8074 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Efficient genetic transformation of sorghum plant requires an effective selection marker system that precisely differentiates transformed cells from non-transformed ones. Hence the present Hygromycin study was undertaken to optimize the selection marker antibiotic Hygromycin to screen transformed cells in sorghum transformation experiments with vectors harbouring hpt gene. Shoot meristem explants of sorghum were grown in somatic embryo induction media containing various concentrations of Hygromycin. At lower concentrations of Hygromycin (20mg/L and less), the growth of shoot apices was unaffected and the survival rates were very high (87.5–100%) during the three sub-culture passages. At a Hygromycin concentration of 50 mg/L the percentage of survival decreased gradually with three subcultures - 62.5, 25 and 0% survival respectively, while at concentrations of 200 and 800 mg/L, these were higher mortalities of explants in the first subculture itself (only 31.3-37.5% survival). The least concentration of Hygromycin at which all susceptible explants died was 50 mg/L (after three subcultures). Therefore it is suggested that 50 mg/L of Hygromycin may be used as the optimum concentration for the selection of sorghum shoot meristem explants transformed with vectors harboring the hpt gene. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | International Journal of Current Research | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Hygromycin, Hpt gene,Transformation,Shoot meristem,Selection,Sorghum. | en_US |
dc.title | Effect of hygromycin on explant selection in genetic transformation experiments of sorghum bicolor (Sorghum bicolor (L.) Moench) | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Journal | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | International Journal of Current Research | en_US |
dc.publication.volumeno | 9(12) | en_US |
dc.publication.pagenumber | 62294-62296 | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | http://www.journalcra.com | en_US |
dc.publication.authorAffiliation | ICAR::Indian Institute of Millets Research | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.naasrating | Not Available | en_US |
Appears in Collections: | CS-IIMilletsR-Publication |
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