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http://krishi.icar.gov.in/jspui/handle/123456789/81598
Title: | A lateral flow assay for the detection of Leptospira lipL32 gene using CRISPR technology |
Other Titles: | Not Available |
Authors: | Natarajan S Joseph J Balamurugan V Estrela P |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | Healthcare Technology Innovation Centre, Indian Institute of Technology Madras, Chennai 600113, India Department of Electrical Engineering, Indian Institute of Technology Madras, Chennai 600036, India ICAR::National Institute of Veterinary Epidemiology and Disease Informatics Department of Electronic and Electrical Engineering, University of Bath, Bath BA2 7AY, UK Centre for Bioengineering & Biomedical Technologies, University of Bath, Bath BA2 7AY, UK |
Published/ Complete Date: | 2023-07-20 |
Project Code: | Not Available |
Keywords: | Leptospira CRISPR dFnCas9 lateral flow device |
Publisher: | MDPI Publishers |
Citation: | Natarajan S, Joseph J, Vinayagamurthy B, Estrela P. A Lateral Flow Assay for the Detection of Leptospira lipL32 Gene Using CRISPR Technology. Sensors. 2023; 23(14):6544. https://doi.org/10.3390/s23146544 |
Series/Report no.: | Not Available; |
Abstract/Description: | The clinical manifestation of leptospirosis is often misdiagnosed as other febrile illnesses such as dengue. Therefore, there is an urgent need for a precise diagnostic tool at the field level to detect the pathogenic Leptospira lipL32 gene at the molecular level for prompt therapeutic decisions. Quantitative polymerase chain reaction (qPCR) is widely used as the primary diagnostic tool, but its applicability is limited by high equipment cost and the lack of availability in every hospital, especially in rural areas where leptospirosis mainly occurs. Here, we report the development of a CRISPR dFnCas9-based quantitative lateral flow immunoassay to detect the lipL32 gene. The developed assay showed superior performance regarding the lowest detectable limit of 1 fg/mL. The test is highly sensitive and selective, showing that leptospirosis diagnosis can be achieved with a low-cost lateral flow device. |
Description: | Not Available |
ISSN: | 1424-8220 |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Sensor |
Journal Type: | Included NAAS Journal List |
NAAS Rating: | 9.90 |
Impact Factor: | 3.90 |
Volume No.: | 23(14) |
Page Number: | 6544 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | https://doi.org/10.3390/s23146544 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/81598 |
Appears in Collections: | AS-NIVEDI-Publication |
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