Phytochemical profiling and nematicidal activity of leaf extracts of Tinospora cordifolia against reniform nematode (Rotylenchulus reniformis)

Abstract

Reniform nematode, Rotylenchulus reniformis is an important nematode associated with castor influencing the productivity of the crop. Efficacy of the leaf extract of Tinospora cordifolia at different concentrations viz., 0.1, 0.5, 1.0, 2.0, 5.0, 10.0 and 20% against the nematode juveniles were evaluated in vitro. The extract, at 20% concentration was recorded to be effective in the reduction of nematode juveniles to 93.8 per cent. The more active non-polar and medium polar compounds which are having melting point less than 200o C were analysed by GC-MS .Eighteen compounds of the total extract were identified of which six compounds were reported to possess nematicidal properties.