Assessing the pathogenicity of Fusarium Oxysporum on soybean using different inoculation techniques

Abstract

Fusarium is one of the important genera of plant parasitic fungi responsible for causing wilt disease in Soybean. The present study was conducted to identify the virulent isolate of the pathogen and to assess their pathogenicity in Soybean cv. Basara. About 10 different Fusarium isolates were collected from the division of Plant Pathology, ICAR- IIOR, Hyderabad, and were assessed for their pathogenicity in Soybean cv. Basara to identify the virulent isolate against the crop using three different pathogenicity tests viz., filter paper method, artificial inoculation method, and sick pot method. Among the 10 different isolates tested for their virulence under three different pathogenicity tests, the Fusarium isolate, CF II was reported to exhibit a higher disease index and wilt incidence in the crop, which has been designated as the virulent isolate against the crop. The pathogenic Fusarium isolate, CF II was characterized using molecular methods based on ITS PCR and confirmed as Fusarium oxysporum.