Pathological studies on experimental infection of buffalo isolate of Trypanosoma evansi infection in Swiss albino mice

Abstract

The study was conducted to evaluate the pathological changes of T. evansi infection in Swiss albino mice inoculated with buffalo isolate. Fifteen Swiss albino mice were used in present experiment divided in two groups consisting of six mice in each group. Group I is considered as a negative (control) group which did not receive any infection. Group II is considered as positive group were inoculated with 1× 104 trypanosomes. Systematic necropsy examination of Group I healthy control mice and Group II infected mice was conducted and histopathological changes were recorded. A part of tissue sample was collected from each visceral organ in 10% NBF for further studies. Gross post mortem examination showed enlargement of spleen and liver. Histopathological tissue section showed presence of clump of T. evansi organisms in brain, lungs, heart, liver, spleen and kidneys. Microscopic examination of brain showed congestion of blood vessels containing trypanosomes. Lung showed congestion haemorrhage, emphysema and broncho-interstitial pneumonia. Heart revealed haemorrhage, congestion and granular appearance of cytoplasm with presence of trypanosome organisms. Liver section showed congestion of blood vessels, hepatocytes were swollen with diffused vacuolar degenerative changes. Spleen revealed haemorrhage in the white pulp with presence of megakaryocytes. Kidney revealed cortico medullary haemorrhage with varying degree of congestion with presence of large number of trypanosome organisms in between the red blood cells.