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http://krishi.icar.gov.in/jspui/handle/123456789/83001
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sridhar Manpal | en_US |
dc.contributor.author | Deepak Kumar | en_US |
dc.date.accessioned | 2024-05-31T12:26:30Z | - |
dc.date.available | 2024-05-31T12:26:30Z | - |
dc.date.issued | 2010-01-01 | - |
dc.identifier.citation | Sridhar Manpal and Deepak Kumar (2010) Production of fibrolytic enzymes in repeat - batch culture using immobilized zoospores of anaerobic rumen fungi. Indian Journal of Biotechnology. 9:87-95 | en_US |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/83001 | - |
dc.description | Not Available | en_US |
dc.description.abstract | The zoospores of two isolates of polycentric rumen fungi, Orpinomyces NIANP 58 (isolated from the faeces of a buffalo) and Anaeromyces NIANP 115 (isolated from the rumen liquor of a cannalulated cow), were immobilized in sodium alginate solution for the production of three fibrolytic enzymes, viz. CMcase, xylanase and β - glucosidase in repeat - batch culture. Enzyme activity was recorded initially (0 h) and after every 24 h up to 72 h of immobilization. CMCellulase activity of 8.5±1.205 units at 0 h in Orpinomyces showed a very steep increase in the activity (598.00±26.87), almost 70 - fold increase at 48 h of immobilization. This increase was reflected in the specific activity as well, while in case of Anaeromyces, an activity of 6.0±0.851 units at 0 h also showed a very steep increase in activity (322.46±1.054 units, recording a 50 - fold increase at 48 h of immobilization). At 0 h, 28.22± 4.556 units of xylanase activity corresponding to a specific activity of 31.36±6.225 were obtained in the case of Orpinomyces, which increased steeply to 955.11±54.93 units at 48 h of immobilization. Thereafter, the activity declined to more or less that obtained at 24 h of immobilization though a high specific activity of 791.20±10.66 was recorded. High xylanase activities were obtained in case of Anaeromyces upon immobilization with activity increasing from 56.88 units at 0 h to as high as 1921 units at 72 h. Anaeromyces isolate yielded 3.85, 4.246, 8.03 and 23.19 units of β - glucosidase, whereas Orpinomyces isolate yielded 6.09, 14.12, 16.63 and 20.89 units of β - glucosidase at 0,24,48 and 72 h, respectively. The results clearly elucidate the feasibility of using the zoospores of anaerobic rumen fungi for large - scale production of these three enzymes, which have great potential in ruminant nutrition in the breakdown of fibrous feeds and also various industrial application | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Not Available | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | zoospores | en_US |
dc.title | Production of fibrolytic enzymes in repeat - batch culture using immobilized zoospores of anaerobic rumen fungi. | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Journal | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Indian Journal of Biotechnology. | en_US |
dc.publication.volumeno | 9 | en_US |
dc.publication.pagenumber | 87-95 | en_US |
dc.publication.divisionUnit | Bioenergetics and Environmental Sciences Division | en_US |
dc.publication.sourceUrl | Not Available | en_US |
dc.publication.authorAffiliation | ICAR::National Institute of Animal Nutrition and Physiology | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
Appears in Collections: | AS-NIANP-Publication |
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