Molecular Profiling of Parents of Some Released Rice Hybrids Using SSR Markers

Abstract

SSR markers targeting (GATA)n motifs are known to be highly polymorphic and useful in many organisms. Twenty eight germplasm of rice (Oryza sativa L.) involved as parents of some of the released hybrids were subjected to polymorphism based on JGT-SSRs analyses to asses the prevalence of genetic diversity. A total of 14 primers of SSRs amplified 37 polymorphic loci. The polymorphic information content ranged from 0.535 (JGT12-8.6) to 0.929 (JGT1–16.2). Prevalence of genetic diversity was revealed by (GATA)n SSRs as range of similarity coefficient value of 0.31 to 1. The parents were grouped by subjecting the similarity values to UPGMA based SAHN clustering. Study revealed the existence of sufficient genetic variation at DNA level in the genotypes being used as parental lines. The results suggested that a relatively less number of (GATA)n microsatellite markers can be used for the reliable estimation of genetic diversity and identification of duplicates.