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http://krishi.icar.gov.in/jspui/handle/123456789/83566| Title: | Characterization of genetic diversity among wild rice accessions using genome specific In-Del markers |
| Other Titles: | Not Available |
| Authors: | Biswaranjan Rout, Sridhar M, Muralidhara B, Kamal Nath Reddy KR, Sundaram RM, Anantha MS, Senguttuvel P, Subba Rao, LV, Padmavathi G, Ranganath HK, Fiyaz AR, Jyothi B, Suvarna Rani C, Kalyani MB, Bidyasagar Mandal and Gireesh C |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Indian Institute of Rice Research |
| Published/ Complete Date: | 2017 |
| Project Code: | Not Available |
| Keywords: | Genetic diversity In-Del markers wild rice species |
| Publisher: | Not Available |
| Citation: | Biswaranjan Rout, Sridhar M, Muralidhara B, Kamal Nath Reddy KR, Sundaram RM, Anantha MS, Senguttuvel P, Subba Rao, LV, Padmavathi G, Ranganath HK, Fiyaz AR, Jyothi B, Suvarna Rani C, Kalyani MB, Bidyasagar Mandal and Gireesh C (2017). Characterization of genetic diversity among wild rice accessions using genome specific In-Del markers. Journal of Rice Research 10 (2): 11-17 |
| Series/Report no.: | Not Available; |
| Abstract/Description: | The genus Oryza contains 22 wild species and two cultivated species namely O. sativa and O. glaberrima. In the present study, 17 accessions belonging to 17 different wild species of rice characterized using 21 wild rice genome specific In-Del markers. A total of 115 fragments/alleles were detected among the 17 wild species. The overall number of alleles per locus ranged from three to 10 with an average of 5.47 indicating the existence of high level of diversity among the accessions. The size of the detected alleles produced using the In-Del primer sets ranged from 105 bp with respect to ch10-044G to 545 bp in ch03-363w. The differences in molecular size between the smallest and the largest allele for a given In-Del locus varied from 25 bp (ch02-342w) to 375 bp (ch05-109G). The PIC values for 21 In-Del loci varied from 0.4 (ch04-276G) to 0.85 (ch05-109G) with an average of 0.68. The overall He values ranged from 0.54 (ch02-342w) to 0.87 (ch05-109G) with an average of 0.72. The cluster analysis grouped the 17 accessions into three main clusters. Cluster I consisted of two subclusters IA with O. glumaepatula, O. longistaminata, O. rufipogon and subcluster IB consist of O. meridionalis, O. barthii, and O. nivara. Cluster II consist of O. rhizomatis, O. officinalis and O. eichingeri which originated in same officinalis complex in rice evolution. Cluster III was subdivided into two sub-clusters with IIIA consisting of O. punctata, O.minuta, O. ridleyi and O. longiglumis and IIIB consisting of O. latifolia, O. australiensis, O. alta and O. grandiglumis. The results of the study reveal that genetic diversity among the accessions from different wild species considered in the present study is moderate. The In-Del markers utilized in this study were able to distinguish 10 out of the 17 species of Oryza. Based on the results obtained it can be concluded that In-Del marker based analysis in consonance with morphological marker data would be more appropriate to ascertain the identity of wild rice. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Journal of Rice Research |
| NAAS Rating: | 0 |
| Impact Factor: | 0 |
| Volume No.: | 10(2) |
| Page Number: | 11-17 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/83566 |
| Appears in Collections: | CS-IIRR-Publication |
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