Effect of Feeding Rice Based Distillers Dried Grains Solubles with and without Enzymes on Gut health of Broiler Chickens

Abstract

A biological experiment was conducted to evaluate the effect of feeding rice based distillers dried grain with solubles (rDDGS) without or with different enzymes on gut health in broiler chickens for 42 days. The experiment was conducted as per 3x4 factorial completely randomized design. A total of 384 broiler chicks were divided into twelve different treatments with 4 replicates for each treatment and each replicate consisted of 8 chicks. Two levels of rDDGS were taken (12.5 and 15%). Protease, xylanase and multienzymes supplementation under different treatments were done. The jejunum histomorphometry in terms of villus height (VH) and their ratio with villus depth (VD) were found significantly (P<0.01) lower at 15% rDDGS level compared to control and 12.5% levels. The xylanase and protease enzyme supplementation significantly (P<0.01) increased the VH and their ratio with VD as compared to control and multienzymes supplemented group. The interaction effect rDDGS and enzyme supplementation was found on VH and VD ratio, where higher ratio was observed at 12.5% rDDGS level with protease enzyme and lower ratio was observed at 15% rDDGS level without enzyme supplementation. The microbiology of the gut in terms of total viable count (TVC) of crop and jejunum significantly (P<0.01) decreased upon increasing rDDGS levels compared to control, whereas reverse effect was observed on Lactobacillus. Thus, it may be concluded that enzymes supplementation improved histomorphometry and microbiology of the gut in broiler chickens at inclusion level of 12.5% rDDGS.