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http://krishi.icar.gov.in/jspui/handle/123456789/8622| Title: | Comparative Assessment of Trypsin Inhibitor vis-à-vis Kunitz Trypsin Inhibitor and Bowman-Birk Inhibitor Activities in Soybean |
| Other Titles: | Not Available |
| Authors: | Vineet Kumar Anita Rani Mohd.Shuaib Priyanka Mittal |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR: Indian Institute of Soybean Research |
| Published/ Complete Date: | 2018 |
| Project Code: | Not Available |
| Keywords: | Trypsin inhibitor Kunitz trypsin inhibitor Bowman-Birk inhibitor ELISA |
| Publisher: | Not Available |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Trypsin inhibitor activity (TIA) in soybean is attributed to two polypeptides, namely, Kunitz trypsin inhibitor (KTI) and Bowman-Birk inhibitor (BBI). Standard spectrophotometric protocol widely followed for estimation of TIA is cumbersome and does not distinguish KTI from BBI. In the present investigation, extraction conditions for KTI were optimized and different forms of this polypeptide were resolved in 180 soybean genotypes of Indian and exotic origin through native PAGE. This led to the identification of three KTI alleles, namely, Tia, Tib, and Tic, with Tia occurring in most of the Indian genotypes. Trypsin-KTI complex assay exhibited binding of Tia polypeptide with 2.51 fold concentration of trypsin. Subsequently, seeds of selected genotypes were subjected to estimation of KTI and BBI activity through densitometry and enzyme-linked immunosorbent assay (ELISA), respectively; and total TIA through standard spectrophotometric protocol. Summation of KTI and BBI was significantly (P < 0.05) lower than that of TIA determined through the spectrophotometric method. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Article |
| Sponsors: | FSSAI |
| Language: | English |
| Name of Journal: | Food Analytical Methods |
| NAAS Rating: | 8.67 |
| Volume No.: | 11(9) |
| Page Number: | 2431-2437 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | doi.org/10.1007/s12161-018-1227-9 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/8622 |
| Appears in Collections: | CS-DSBR-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| kumar2018 FOOD ANALYTICAL.pdf | 537.47 kB | Adobe PDF | View/Open |
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