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http://krishi.icar.gov.in/jspui/handle/123456789/8879
Title: | Serological detection of Grapevine leafroll-associated virus 4 in grapevine growing areas of India using polyclonal antiserum raised against the recombinant coat protein |
Other Titles: | Not Available |
Authors: | Richa Raia, S.M.Paul Khurana, Sandeep Kumar, Nitika Gupta, V.K. Baranwala |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | Indian Agricultural Research Institute, New Delhi ICAR-Directorate of Floricultural Research Amity Institute of Biotechnology, Amity University Orissa University of Agriculture and Technology, Bhubaneswar |
Published/ Complete Date: | 2018-03-30 |
Project Code: | Not Available |
Keywords: | GLRaV-4 ACP-ELISA DIBA DAS-ELISA |
Publisher: | Not Available |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Grapevine leafroll disease (GLD), caused by a complex of serologically and genetically distinct or related viral species termed as Grapevine leafroll associated viruses (GLRaVs), is a serious threat to the grape production worldwide. Recently, Grapevine leafroll-associated virus 4 (GLRaV-4) has been reported in the Indian vineyards, mostly as a mixed infection with GLRaV-3 and GLRaV-1. In order to screen the planting material, a polyclonal antiserum against the in vitro expressed coat protein (CP) of GLRaV-4 was produced. The antiserum, purified IgGs and conjugated IgGs detected GLRaV-4 in crude saps of infected grapevines and purified coat protein when used in antigen coated plate enzyme-linked immunosorbent assay (ACP-ELISA), double antibody sandwich enzyme- linked immunosorbent assay (DAS-ELISA), Western blotting and dot immunobinding assay (DIBA). GLRaV-4 could be detected in 54% (39 out of 72) of symptomatic grapevine samples from different grape growing regions of India using DAS-ELISA. The validation of DAS-ELISA results was done by RT-PCR using GLRaV-4 CP-specific primers. To the best of our knowledge, this is the first report of a polyclonal antiserum against the in vitro expressed CP of GLRaV-4 and its successful application in diagnosis of the virus in grape vineyards of India |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Journal |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Crop Protection |
NAAS Rating: | 8.38 |
Volume No.: | 109 |
Page Number: | 128–135 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/8879 |
Appears in Collections: | HS-DFR-Publication |
Files in This Item:
File | Description | Size | Format | |
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Crop protection.pdf | 1.6 MB | Adobe PDF | View/Open |
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