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http://krishi.icar.gov.in/jspui/handle/123456789/8991| Title: | Sequencing and analysis of invariant surface glycoprotein (ISG) gene from Trypanosoma evansi dog isolate |
| Other Titles: | Not Available |
| Authors: | Rudramurthy GR Sengupta PP Lakkundi JN Ligi M D'Souza PE Rahman H |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Institute of Veterinary Epidemiology and Disease Informatics |
| Published/ Complete Date: | 2015-01-01 |
| Project Code: | Not Available |
| Keywords: | Trypanosomosis Dog isolate T.evansi ISG-75 Phylogenetic analysis |
| Publisher: | Research in Biotechnology |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Trypanosoma (T) evansi causes a chronic wasting disease called “surra” in cattle, buffaloes, horses, canines, felines etc. In the present study T.evansi isolated from dog from Karnataka state in India was used to sequence invariant surface glycoprotein (ISG) gene. The sequence obtained was analyzed to elucidate its relationship with other isolates/ species. The ISG gene sequences obtained from four recombinant clones revealed the open reading frame (ORF) of 1521 nucleotide (nt) encoding a polypeptide of 506 amino acids (aa) and belongs ISG-75 gene family. Sequence analysis revealed respectively, 92-99% and 65-99% similarity at nucleotide and amino acid levels, with other isolates/ species. Hence, the present studied T.evansi isolate belongs to the RoTat 1.2 strain. |
| Description: | Not Available |
| ISSN: | 2229-791X |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Research in Biotechnology |
| Volume No.: | 6 |
| Page Number: | 1-9 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | http://updatepublishing.com/journal/index.php/rib/issue/view/272 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/8991 |
| Appears in Collections: | AS-NIVEDI-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 392-1457-1-PB1.pdf | 374.17 kB | Adobe PDF | View/Open |
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