Expression of glycoprotein gene of rabies virus and evaluation of recombinant protein for seromonitoring of vaccinal antibodies in dogs

Abstract

Rickettsia felis bacterium has been observed since the early twentieth century in the cat flea. It was grown for the first time in my laboratory on cell Xenopus then insect cells particularly Aedes albopictus cells. It grows only at temperature under 30 ◦C. First described in California, the bacterium is present in the whole world. For a long time it was considered a rare disease associated with fleas. In fact, in recent years, Rickettsia felis was found as the most frequently identified bacteria in patients with fever in tropical countries after malaria. These data were confirmed in West Africa and East Africa by different teams. Rickettsia felis has also been detected in tropical and tropical areas of South America, Asia and Oceania. Rickettsia felis and is the most common rickettsial currently in the world and very frequently associated with fever in the tropics and cough but no rash. In contrast controls without fever, can cause at a lower but significant level, the presence of Rickettsia felis DNA. So these circulations of Rickettsia felis in the blood that are not always associated with fever. Possible vectors in tropical areas may be mosquitoes in Africa. Rickettsia felis has the same epidemiological distribution as malaria. Rickettsia felis was found in Anopheles species and Aedes albopictus. A mouse experimental model was established showing that Rickettsia felis could be vectorized by Anopheles gambiae. Rickettsia felis, finally, recently, was found in book lice in the dust, which further complicates the epidemiological cycle of this bacterium. R. felis may be the most common bacteria emerging virus (RABV) dependent and laborious Rapid Fluorescent Focus Inhibition Test (RFFIT) is employed in selected laboratories in India to monitor the vaccinal antibodies. This situation demands an alternative rapid, sensitive, specific and user friendly test. Hence, this study was undertaken to express the recombinant glycoprotein (rRVL-G) of Dr. Larghi’s strain of RABV and evaluate it’s diagnostic potential.